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목차 1
새조개 (Fulvia mutica)의 Microsatellite Markers 개발을 위한 차세대 염기서열 분석법의 활용 = Utilization of next-generation sequencing for the development of microsatellite markers in the egg cockle (Fulvia mutica) / 동춘매 ; 노은수 ; 이미난 ; 김우진 ; 김영옥 ; 김은미 1
ABSTRACT 1
서론 1
재료 및 방법 2
1. 시료 확보 및 genomic DNA 추출 2
2. 차세대 염기서열 분석 (NGS) 및 microsatellite 탐색 3
3. Microsatellite 마커 선별 3
4. Microsatellite 마커의 효율성 검증 3
결과 및 고찰 4
1. DNA 품질 검증 및 라이브러리 제작 4
2. Microsatellite 탐색 및 다형성 확인 4
3. Microsatellite 마커 제작 7
4. Microsatellite 마커의 효율성 검증 8
요약 9
REFERENCES 9
| 번호 | 참고문헌 | 국회도서관 소장유무 |
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| 3 | Barinova, A.A., Kumagai, K., Nakajima, M., and Taniguchi, N. (2002) Identification and characterization of microsatellite DNA markers developed in threeline grunt Parapristipoma trilineatum. Suisannzousyoku, 32: 27-32. | 미소장 |
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| 8 | Dong, C.M., Lee, H.M., Lee, M.N., Noh, E.S., Nam, B.H., Kim, Y.O., and Kim, E.M. (2022) Development of new Microsatellite DNA Markers Using Next-generation Sequencing in pacific oyster Crassostrea gigas. Korean Journal of Malacology, 38(3): 129-138. | 미소장 |
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| 12 | Gardner, M.G., Fitch, A.J., Bertozzi, T., and Lowe, A.J. (2011) Rise of the machines-recommendations for ecologists when using next generation sequencing for microsatellite development. Molecular Ecology Resources, 11(6): 1093-1101. | 미소장 |
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| 14 | Inoue, T. (1955) Ecological studies of Fulvia mutica (Reeve)-Ⅱ Spawning season. Bulletin of the Japanese Society of Scientific Fisheries. 21(1): 27-29. | 미소장 |
| 15 | Kalinowski, S.T., Taper, M.L., and Marshall, T.C. (2007)Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Molecular Ecology, 16(5):1099-1106. | 미소장 |
| 16 | Kandeel, K.E., Mohammed, S.Z., Mostafa, A.M., and Abd-Alla, M.E. (2017) Population Dynamics of the Cockle Cerastoderma glaucum: A comparison between Lake Qarun and Lake Timsah, Egypt. Turkish Journal of Fisheries and Aquatic Sciences, 17:945-958. | 미소장 |
| 17 | Kim, T.H., Kim, Y.K., Son, J.H., Chun, J.B., and Yoon, Y.M. (2021) Utilization of whole genome re-sequencing for large-indel markers development in malting barley. Korean Journal of Breeding Science, 53(3): 266-276. | 미소장 |
| 18 | Kim, K.S., Noh, C.H., Sade, A., and Bang, I.C. (2015)Effectiveness of microsatellite markers for parentage analysis of giant grouper (Epinephelus lanceolatus)broodstock. Korean Journal of lchthyology, 27(1):10-15. | 미소장 |
| 19 | Kim, K.S., Ha, B.S., Bae, T.J., Jin, J.H., and Kim, H.J. (1993a) Comparision of Food Components in the Raw, Cooked Meat and Cooked Meat Extracts of Cockle Shell 1. Proximate compositions and lopid components. Bulletin of the Korean Fisheries Society, 26: 102-110. | 미소장 |
| 20 | Kim, K.S., Ha, B.S., Bae, T.J., Jin, J.H., and Kim, H.J. (1993b) Comparision of Food Components in the Raw, Cooked Meat and Cooked Meat Extracts of Cockle Shell 2. Nitrogenous compounds and minerals. Bulletin of the Korean Fisheries Society, 26: 111-119. | 미소장 |
| 21 | Kim, S.G., Morishima, K., Satoh, N., Fujioka, T., Saito, S., and Arai, K. (2007) Parentage assignment in hatchery population of brown sole Pleuronectes herzensteini by microsatellite DNA markers. Fisheries Science, 73: 1087-1093. | 미소장 |
| 22 | Koren, S., Walenz, B.P., Berlin, K., Miller, J.R., Bergman, N.H., and Phillippy, A.M. (2017) Canu:scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation. Genome research, 27(5): 722-736. | 미소장 |
| 23 | KOSIS (2020) Fishery Production Trend Survey Report. Korean statistical information service. Retrieved April 19, 2021, from http://kosis.kr. | 미소장 |
| 24 | Mardis, E.R. (2008) Next-generation DNA sequencing methods. Annual Review of Genomics and Human Genetics, 9: 387-402. | 미소장 |
| 25 | Martínez, L., Arias, A., Méndez, J., Insua, A., and Freire, R. (2009) Development of twelve polymorphic microsatellite markers in the edible cockle Cerastoderma edule (Bivalvia: Cardiidae). Conservation Genetics Resources, 1: 107-109. | 미소장 |
| 26 | Matsuoka, Y., Tanaka, S., and Ikuta, T. (1968) Technical study on seed production of Fulvia mutica. Contributions from Fisheries Station, Kyoto Prefecture, 31: 13-27. | 미소장 |
| 27 | Min, D.K., Lee, J.S., Koh, D.B., and Je, J.G. (2004)Mollusks in Korea. Min Molluscan Research Institute, Hanguel Graphics, Busan. p. 433. | 미소장 |
| 28 | Nishihiro, T. (1980) Studies on the artificial production of cockle, Fulvia mutica, 1: Inducing spawning and the early development. Bulletin of the Kyoto Institute of Oceanic and Fishery Science, 4: 13-17. | 미소장 |
| 29 | Norris, A.T., Bradley, D.G., and Cunningham, E.P. (2000)Parentage and relatedness determination in farmed Atlantic salmon (Salmo salar) using microsatellite markers. Aquaculture, 182: 73-83. | 미소장 |
| 30 | Oosterhout, C.V., Hutchinson, W.F., Wills, D.P.M., and Shipley, P. (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes, 4(3):535-538. | 미소장 |
| 31 | Paetkau, D. (1999) Microsatellites obtained using strand extension: An enrichment protocol. Biotechniques, 26(4): 690-697. | 미소장 |
| 32 | Park, C.J., Nam, W.S., Lee, M.S., Kang, J.Y., and Kim, K.K. (2014) Microsatellite multiplex PCR method for selective breeding studies in Pacific abalone (Haliotis discus hannai). Korean Journal of Malacology, 30(4):383-390. | 미소장 |
| 33 | Pinggera, A., Mackenroth, L., Rump, A., Schallner, J., Beleggia, F., Wollnik, B., and Striessnig, J. (2017)New gain-of-function mutation shows CACNA1D as recurrently mutated gene in autism spectrum disorders and epilepsy. Human Molecular Genetics, 26(15): 2923-2932. | 미소장 |
| 34 | Rudnick, J.A., and Lacy, R.C. (2008) The impact of assumptions about founder relationships on the effectiveness of captive breeding strategies. Conservation Genetics, 9(6): 1439-1450. | 미소장 |
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| 36 | Song, W.H., and Chung, S.M. (2019) Whole genome re-sequencing and development of SSR markers in oriental melon. Journal of Plant Biotechnology, 46(2):71-78. | 미소장 |
| 37 | Untergrasser, A., Cutcutache, I., Koressaar, T., Ye, J., Faircloth, B.C., Remm, M., and Rozen, S.G. (2012) Primer3 - new capabilities and interfaces. Nucleic Acids Research, 40(15): e115. | 미소장 |
| 38 | Yang, H.S., Kang, D.H., Park, H.S., and Choi, K.S. (2011)Seasonal Changes in Reproduction and Biochemical Composition of the Cockle, Fulvia mutica Reeve (1884), in Cheonsu Bay off the West Coast of Korea. Journal of Shellfish Research, 30(1): 95-101. | 미소장 |
| 39 | Yu, H., Xie, W., Wang, J., Xing, Y., Xu, C., Li, X., Xiao, J., and Zhang, Q. (2011) Gains in QTL detection using an ultra-high density SNP map based on population sequencing relative to traditional RFLP/SSR markers. PLoS One, 6(3): e17595. | 미소장 |
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