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22대 대한민국 국회 국회정보길라잡이 국회의원검색
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초록보기

The efficiency of protein extraction from Hongjam, a steamed mature silkworm, was quantitatively evaluated using various chemical buffers and physical methods. This study considers the difficulty of protein extraction yield due to the high content of hydrophobic amino acids in Hongjam compared to 5th instar-3rd day silkworm larvae. Results indicated that urea buffer enhanced protein yield more effectively than RIPA buffer. Additionally, the application of physical methods such as microwave treatment to samples treated with RIPA buffer increased yields by up to 22%, achieving concentrations as high as 3.9 mg/mL. Circular dichroism (CD) analysis showed that proteins extracted with urea buffer retained their structural integrity, exhibiting deeper and more prominent peaks associated with random coil structure. In addition, physical methods such as vortexing, sonication, microwave and homogenization increased the extraction yield of larger molecules without altering protein structures, suggesting their potential scalability for industrial applications. These results demonstrate the critical role of selecting appropriate extraction methods to optimize the yield and functionality of proteins from Hongjam, with implications for its use in biotechnological applications and nutraceuticals.

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기사명 저자명 페이지 원문 목차
Insect-originated functional food : nutritional benefits and applications Yoon-Jo Lee, HaeYong Kweon, You-Young Jo, Seong-Gon Kim p. [1-8, 실은 99-106]
Comparison of chemical and physical extraction methods of steamed-mature silkworm (Hongjam) protein Ji Hae Lee, Jong Woo Park, Seong-Wan Kim, Sang Kuk Kang, Seul Ki Park, Hyeok Gyu Kwon, Seong Ryul Kim p. [1-7, 실은 107-113]
Discovery of the vagrant darter, Sympetrum vulgatum (Odonata: Libellulidae) in Korea Jeong Sun Park, Jong Moon Kim, Hyeong Gi Jeon, Sung Soo Kim, Jee-Young Pyo, Iksoo Kim p. [1-10, 실은 114-123]
Field monitoring and population genetic analysis of the dragon swallowtail Sericinus montela (Lepidoptera: Papilionidae), a vulnerable species in South Korea Seung Hyun Lee, Jeong Sun Park, Jee-Young Pyo, Sung-Soo Kim, Heon Cheon Jeong, Iksoo Kim p. [1-15, 실은 124-138]
Application and evaluation of LAMP-PCR for the diagnosis of silkworm pebrine disease Jong Woo Park, Pu Reun Kook, Jeong Sun Park, Yeong Hee Cho, Seul Ki Park, Hyeok Gyu Kwon, Ji Hae Lee, Sang Kuk Kang, Seong-Wan Kim, Kee Young Kim, Seong-Ryul Kim p. [1-8, 실은 139-146]
Analyzing the nutritional value of Protaetia brevitarsis larvae feeding on coffee and oyster mushroom cultivation by-products Min Ji Park, Hyung Joo Yoon, Sun Young Kim p. [1-9, 실은 147-155]
Comparative analysis of Bombyx batryticatus and Bombyx mori on α-glucosidase inhibition and their bioactive compositions Hwa Lee, Jong-Hoon Kim p. [1-7, 실은 156-162]

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