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CONTENTS

Contributors XV

Preface XXIII

Volumes in Seeries xxv

1. Water Compartments in Cells 1

2. Yeast Osmoregulation 29

3. Physiological Analysis of Bacterial Mechanosensitive Channeles 47

4. Control of Cell Cycle in Response to Osmostress: Lessons from Yeast 63

5. Bacterial Osmosensing Transporters 77

6. Plant Gene Networks in Osmotic Stress Response: From Genes to Regulatory Networks 109

7. Osmosensing by Integrins in Rat Liver 129

8. Hyperosmotic Activation of the CD95 System 145

9. New Approaches for Determining Apoptotic Volume Decrease in Cells 161

10. Transient Receptor Potential Channels in Mechanosensing and Cell Volume Regulation 183

11. Cell Volume Regulatory Ion Channels in Cell Proliferation and Cell Death 209

12. Actin Cytoskeleton Architecture and Signaling in Osmosensing 227

13. Osmotic Stress and DNA Damage 241

14. Transcriptional Activator TonE-Binding Protein in Cellular Protection and Differentiation 253

15. Desiccation Response of Mammalian Cells: Anhydrosignaling 269

16. Tonicity-Regulated Gene Expression 279

17. Hyperosmotic Induction of Mitogen-Activated Protein Kinase Scaffolding 297

18. Osmoregulation of Bile Formation 313

19. Osmosignaling and Volumen Regulation in Intestinal Epithelial Cells 325

20. Osmotic Regulation of Cellular Glucose Uptake 343

21. Effects of Osmolytes on Protein Folding and Aggregation in Cells 355

22. Simulations of Macromolegules in Protective and Denaturing Osmolytes: Propertiees of Mixed Solvent Systems and Their Effects on Water and Protein Structure and Dynamics 373

23. Application of the Transfer Model to Understand How Naturally Occurring Osmolytes Affect Protein Stability 397

24. Mechanisms of High Salinity TOlerance in Plants 419

25. Phenotype of the Taurine Transporter Knockout Mouse 439

26. Molecular Basis of Osmolyte Effects on Protein and Metabilites 459

27. Methods of Changing Biopolymenr Volumen Fraction and Cytoplasmic Solute Concentrations for In Vivo Biophysical Studies 487

28. Characterization of Plant Aquaporins 505

Author Index 533

Subject Index 571

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For over fifty years the Methods in Enzymology series has been the critically acclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume features articles on the topic of osmosensing and osmosignaling written by experts in the field.