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국회도서관 홈으로 정보검색 소장정보 검색

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목차

I. 서론 6

II. 실험 8

1. 재료 8

1) 동물 8

2) 약재 8

3) 시약 및 기기 9

2. 방법 10

1) 약침액의 제조 10

2) 세포독성측정 12

3) 신장염 동물 모델 12

4) 실험군 분류 및 처치 13

5) 신장염 평가 14

6) 통계처리 15

III. 성적 16

1. 목통 약침액의 세포독성 16

2. LPS 투여에 따른 TNF-α의 변화 17

1) Serum TNF-α 17

2) Renal TNF-α 18

3. 목통 약침이 LPS에 의한 rat의 신장염에 미치는 영향 19

1) 혈액학적 분석 19

2) Blood chemistry 22

3) 소변 분석 26

4) 신장 내 MPO(myeloperoxidase)활성 29

5) 조직학적 분석 31

IV. 고찰 32

V. 결론 38

參考文獻 39

Abstract 42

List of Tables

Table 1. Reagents 9

Table 2. Instruments 10

Table 3. Blood cell counts in LPS-stimulated rats. 19

Table 4. Blood chemistry analysis in LPS-stimulated rats. 22

Table 5. Unine analysis in LPS-stimulated rats. 26

Table 6. Inflammatory activity in kidney of LPS-stimulated rats. 29

List of Figures

Figure 1. Cytotoxicity of AL-HAS on rat liver cells. 16

Figure 2. Effect of LPS on serum TNF-α level in SD rats. 17

Figure 3. Effect of LPS on renal TNF-α level in SD rats. 18

Figure 4. Effect of AL-HA on WBC countin blood of LPS-stimulated rats. 20

Figure 5. Effect of AL-HA on neutrophil count in blood of LPS-stimulated rats. 21

Figure 6. Effects of AL-HA on serum BUN level in LPS-stimulated rats. 23

Figure 7. Effects of AL-HA on serum TNF-α level in LPS-stimulated rats. 24

Figure 8.Effects of AL-HA on serum CINC-1 leve lin LPS-stimulated rats. 25

Figure 9. Effect of AL-HA on urinary volume in LPS-stimulated rats. 27

Figure 10. Effects of AL-HA on total protein level in urine of LPS-stimulated rats. 28

Figure 11. Effects of AL-HA on renal MPO level in LPS-stimulated rats. 30

Figure. 12. Histological analysis of renal tissue in LPS-stimulated rats.(H&E stain) 31

List of Schemes

Scheme 1. Manufacturing procedure of Akebiae Lignum Herbal acupuncture Solution. 11

Scheme 2. Experimental Procedure 13