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Eight Bacillus thuringiensis strains activated against mosquito larva were compared their characterization. Spherical-shaped parasporal inclusion of B. thuringiensis subsp. tohokuensis CAB167 was observed by phase-contrast microscopy and scanning electron microscopy. LC50 values of B. thuringiensis subsp. tohokuensis CAB167 against Culex pipiens molestus, Culex pipiens pallens, and Aedes aegyti were 173, 190 and 580 ng/ml, respectively. B. thuringiensis subsp. tohokuensis CAB167 had a parasporal
inclusion containing 4 major protein components, for example, 135, 80, 49 and 28-kDa by SDS-PAGE.
Otherwise, after trypsin digestion of parasporal inclusion, SDS-PAGE was showed new protease-resistant peptides at 72 and 63-kDa. Activated toxins of isolated CAB167 were different from other reference strains on a serological by immuno-diffusion test.
본 연구는 모기에 활성을 나타내는 Bacillus thuringiensis subsp. tohokuensis CAB167균주에서 생산된 내독소결정단백질의 특성을 조사하였다.
B. thuringiensis subsp. tohokuensis CAB167균주는 위상차 현미경과 주사전자현미경으로 관찰하여 일반적으로 모기에 활성을 나타내는 spherical type의 내독소결정성단백질을 형성하는 것으로 나타났다. 국내에서 서식하는 지하집모기(Culex pipiens molestus), 빨간집모기(Culex pipiens pallens), 이집트숲모기(Aedes aegyti)에 대한 이 균주의 살충활성검정은 각각 173, 190, 580 ng/ml의 LC50 값으로 각각 활성을 보였다. B. thuringiensis subsp. tohokuensis CAB167균주에서
생산된 내독소결정성단백질의 SDS-PAGE를 통해 135, 80, 49와 28-kDa의 주요한 4개의 밴드를 나타냈다.
내독소결정성단백질의 소화에 영향을 미치는 곤충중장액과 유사한 trypsin효소를 처리하여 72와 63-kDa 의 단백질이 새롭게 생성하였다. 파리목 위생해충인 모기에 활성을 나타내는 다른 7개의 B. thuringiensis 균주들의 내독소 결정성단백질과 혈청학적인 비교실험에서 독소단백질이 차이가 있는 것을 확인하였다.번호 | 참고문헌 | 국회도서관 소장유무 |
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1 | Benintende, G.B., (2000) Characterizaion of INTA 51-3, a new atypical strain of Bacillus thuringiensis from Argentina, Curr. Microbiol | 미소장 |
2 | BACILLUS THURINGIENSIS USE IN AGRICULTURE: A MOLECULAR PERSPECTIVE ![]() |
미소장 |
3 | Chilcott, C.N., (1994) Opportunities for finding new Bacillus thuringiensis sratin collection, Appl. Eviron. Microbiolo | 미소장 |
4 | Bioassay of Environment-friendly Insecticides for Management of Mosquito, Culex pipiens molestus | 소장 |
5 | Bioactive Characterization of Bacillus thuriniensis subsp. kurstaki CAB133 Isolated from Domestic Soil | 소장 |
6 | Crickmore, N., (1998) Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins, Microbiol. & Mol. Biolo. Rev | 미소장 |
7 | Interaction of Bacillus thuringiensis svar. israelensis Cry toxins with binding sites from Aedes aegypti (Diptera: Culicidae) larvae midgut ![]() |
미소장 |
8 | Dulmage, H.T., (1970) Insecticidal activity of HD-1, a new isolate of Bacillus thuringiensis var. alesti, J. Inverte. Pathol | 미소장 |
9 | Insecticidal Bacterial Proteins Identify the Midgut Epithelium as a Source of Novel Target Sites for Insect Control ![]() |
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10 | Federici,B.A., (1990) The parasporal body of Bacillus thuringiensis subsp. israelensis: structure, protein composition and toxicity, Rutgers University Press | 미소장 |
11 | Glare, T.R., (2000) Bacillus thuringiensis: Biology, ecology and safety, Wiley | 미소장 |
12 | Bio/Technology Stock Index ![]() |
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13 | The 23-Kilodalton CytB Protein is Solely Responsible for Mosquito Larvicidal Activity on Bacillus thuringlensis serovar kyushuensis ![]() |
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14 | Khodyrev, V.P., (2006) Characterizaion of crystal-forming bacteria Bacillus thuringiensis subsp. tohokuensis toxic to mosquite larvae, Biolo. Bull | 미소장 |
15 | Distribution and Characterization of Bacillus thuringiensis isolated from soils in Korea ![]() |
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16 | Distribution and Characterization of Bacillus thuringiensis isolated from soils in Korea ![]() |
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17 | Characterization of Bacillus thuringiensis isolated in Granary Dusts ![]() |
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18 | Isolation and Activity of Insect Pathogenic Bacillus thuringiensis Strain from Soil | 소장 |
19 | Toxicity of Bacillus thuringiensis subsp. israelensis to adult Aedes aegypti mosquitoes. ![]() |
미소장 |
20 | Cloning and Characterization of a Novel Bacillus thuringiensis Cytolytic Delta-Endotoxin ![]() |
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21 | Cleavage of structural proteins during the assembly of the head of bacteriophage T4. ![]() |
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22 | Physiology of sporeforming bacteria associated with insects: minimal nutritional requirements for growth, sporulation, and parasporal crystal formation of Bacillus thuringiensis. ![]() |
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23 | Mizuki, E., (1999) Unique activity associated with non-insecticidal Bacillus thuringiensis parasporal inclusions: in vitro cell-killing action on human cancer cell, Appl. Microbiolo | 미소장 |
24 | Cloning and Characterization of Two Novel Genes, cry24B and s1orf2, from a Mosquitocidal Strain of Bacillus thuringiensis serovar sotto ![]() |
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25 | Occurrence of two pathotypes in Bacillus thuringiensis subsp. fukuokaensis (flagellar serotype 3a:3d:3e) ![]() |
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26 | Ohba, M., (1992) Unique isolate of Bacillus thuringiensis serovar japonensis with a high larvicidal activity specified for scarabaeid beetles, Lett. Appl. Microbiol | 미소장 |
27 | Ohba, M., (1995) Bacillus thuringiensis serovar higo (flagellar serotype 44), a new serogroup with a larvicidal activity preferential for the anopheline mosquito, Letter in appl. Micro | 미소장 |
28 | A new subspecies of Bacillus thuringiensis isolated in Japan: Bacillus thuringiensis subsp. tohokuensis (serotype 17) ![]() |
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29 | Ohgushi, A., (2003) Characterizaion of mosquiocidal Bacillus thuringiensis serovar sotto strain isolated from Okinawa, Jap. J. Appl. Microbiol | 미소장 |
30 | Isolation of a Bacillus thuringiensis strain (serotype 8a:8b) highly and selectively toxic against mosquito larvae ![]() |
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31 | Wasano, N., (1998) Delta-endotoxin proteins associated with spherical parasporal inclusions of the four Lepidoptera -specific Bacillus thuringiensis strains, Appl. Micro | 미소장 |
32 | WHO, (2005) Guidelines for lavoratory and field testing of mosquito larvicides. World Health Organizaion communicable disease control, prevention and eradication, WHO pesticide evaluation scheme | 미소장 |
33 | Synergistic effects of the 65- and 25-kilodalton proteins of Bacillus thuringiensis strain PG-14 (serotype 8A:8B) in mosquito larvicidal activity. ![]() |
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34 | Characterization of mosquitocidal activity of Bacillus thuringiensis subsp. fukuokaensis crystal proteins. ![]() |
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35 | Characterization of a Bacillus thuringiensis δ-Endotoxin Which Is Toxic to Insects in Three Orders ![]() |
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36 | Purification and immunological characterization of particular delta-endotoxins from three strains of Bacillus thuringiensis ![]() |
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