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국내 토양으로부터 파밤나방에 활성있는 Bacillus thuringiensis subsp. kurstaki KB100균주를
분리 동정하였다. 난방제 해충인 파밤나방에 대한 B. thuringiensis의 살충력을 강화시키기 위해 몇 개의
protease inhibitor를 조사하였으며, 이 중 단백질 분해 억제 능력이 가장 좋은 tannic acid을 선발하였다.
B. thuringiensis의 배양액에 tannic acid을 혼합 처리하여 파밤나방 2령 유충을 대상으로 생물활성을
조사한 결과, B. thuringiensis 단독처리는 54.4%의 사충율을 보인 반면 B. thuringiensis와 4 mM tannic
acid을 혼합하여 처리하면 64.0% 그리고 B. thuringiensis+40 mM tannic acid는 95.5%의 높은 상승효과를각각 나타냈다. 그러나 B. thuringiensis+80 mM tannic acid 에서는 53.3%로 단독처리의 경우 보다 사충율이
낮게 나타났다. 3령 유충에서도 B. thuringiensis 단독처리는 60.0%였으며 B. thuringiensis+40 mM tannic
acid는 93.3%로 사충율이 증가하는 상승효과를 보였다. 야외포장 실험에서 B. thuringiensis 단독처리는
1회, 2회, 3회 누적 처리에서 각각 61.8%, 80.4% 그리고 47.3%의 사충율을 나타낸 것에 비해 B. thuringiensis
와 40 mM tannic acid의 혼합 처리구에서는 83.9%, 89.4%와 66.8%로 사충율이 증가되었다.
Bacillus thuringiensis subsp. kurstaki KB100 isolated from the domestic soil have the
most effective activity against the beet armyworm, Spodoptera exigua larva. The tannic acid as protease
inhibitor might be increased the efficacy of sublethal concentrations of B. thuringiensis. The tannic acid
was identified as a protease inhibitor that could increased the efficacy of sublethal concentrations of
B. thuringiensis. Mixture of B. thuringiensis and tannic acid was investigated the mortality of S. exigua
larva in the laboratory and field. When B. thuringiensis treated to 2nd larva of S. exigua, mortality
was shown 54.4%. However, mixtures of B. thuringiensis with 4 and 40 mM tannic acid were increased
mortalities to 2nd larva of S. exigua as 64.0 and 95.5%, respectively. Also, synergy effect of mixture
of B. thuringiensis and 40 mM tannic acid was increased the mortality of S. exigua 3rd larva to 93.3%,
even though 60.0% mortality with only B. thuringiensis treatment. On the other hand, the mortality of
mixture with B. thuringiensis and 80 mM tannic acid was 53.3% lower than B. thuringiensis single
treatment. In the welsh onion field, the accumulated mortalities of 3 times replicated with mixture of
B. thuringiensis and 40 mM tannic acid were 83.9, 89.4 and 66.8% compare with 61.8, 80.4 and 47.3%
as only B. thuringiensis treatment, respectively.번호 | 참고문헌 | 국회도서관 소장유무 |
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1 | Ahn, S.B., I.S. Kim, W.S. Cho, M.H. Lee and K.M. Choi. 1989. The Occurance of the crop insect pests from Korea in 1988. Korean J. Appl. Entomol. 28(4): 246-253. | 미소장 |
2 | Feeding Stimulants for the European Corn Borer (Lepidoptera: Pyralidae): Additives to a Starch-Based Formulation for Bacillus thuringiensis ![]() |
미소장 |
3 | A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding ![]() |
미소장 |
4 | Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. ![]() |
미소장 |
5 | Influence of bait formulations on the effectiveness of Bacillus thuringiensis against Spodoptera littoralis (Boisd.) (Lep., Noctuidae) ![]() |
미소장 |
6 | Eveleens, K.G., R. Van Den Bosch and L.E. Ehler. 1973. Secondary outbreak induction of beet armyworm by experimental insecticide application in cotton in California. Entomol. 2: 497-503. | 미소장 |
7 | Finney, D.J. 1971. Probit analysis, estimation of the median effective dose. Cambridge University Press. London. pp 19-47. | 미소장 |
8 | Goh, H.G., J.D. Park, Y.M. Choi, K.M. Choi and I.S. Park. 1991. The host plants of beet armyworm, Spodoptera exigua (Hübner), (Lepidoptera: Noctuidae) and its occurrence. Korean J. Appl. Entomol. 30(2): 111-116. | 미소장 |
9 | Goh, H.G., J.S. Choi, K.B. Uhm, K.M. Choi and J.W. Kim. 1993a. Spatial distribution pattern of beet armyworm, Spodoptera exigua (Hübner), larvae in the welsh onion field. Korean J. Appl. Entomol. 32(2): 134-138. | 미소장 |
10 | Goh, H.G., J.S. Choi, K.B. Uhm, K.M. Choi and J.W. Kim. 1993b. Seasonal fluctuation of beet armyworm, Spodoptera exigua (Hübner), adult and larva. Korean J. Appl. Entomol. 32(4): 389-394. | 미소장 |
11 | Goh, H.G., S.G. Lee, B.P. Lee, K.M. Choi and J.H. Kim. 1990. Simple mass-rearing of beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae), on an artificial diet. Korean J. Appl. Entomol. 29: 180-183. | 미소장 |
12 | Characterization of New Bacillus thuringiensis Isolated with Bioactivities to Tobacco Cutworm, Spodoptera litura (Lepidoptera: Noctuidae) | 소장 |
13 | Kim, H.S., D.W. Lee, H.W. Park, Y.M. Yu, J.I. Kim and S.K. Kang. 1995a. Distribution and characterization of Bacillus thuringiensis isolated form soils of sericultural farms in Korea. Korean J. Seric. Sci. 34(1): 57-61. | 미소장 |
14 | Kim, K.C., J.D. Park and D.S. Choi. 1995b. Seasonal occurrence of Spodoptera exigua in Chonnam province and a possibility of their control in vinyl house with pheromone trap. Korean J. Appl. Entomol. 34(2): 106-111. | 미소장 |
15 | Kim, Y. and N. Kim. 1997. Cold hardiness of the beet armyworm, Spodoptera exigua (Noctuidae: Lepidoptera). Environ Entomol. 26: 1117-1123. | 미소장 |
16 | Cleavage of structural proteins during the assembly of the head of bacteriophage T4. ![]() |
미소장 |
17 | Luo, L.Z., Y.Z. Cao and X.F. Jiang. 2000. The study on occurrence characteristics and trend of beet armyworm. Plant Protection. 26(3): 37-39. | 미소장 |
18 | Potentiation of Bacillus thuringiensis insecticidal activity by serine protease inhibitors ![]() |
미소장 |
19 | Opprert, B. 1999. Protease interaction with Bacillus thurigiensis insecticidal toxin. Arch. Insect Biochem. Physiol. 42: 1-12. | 미소장 |
20 | Park, J.D., H.G. Goh, J.H. Lee, W.J. Lee and K.J. Kim. 1991. Flight activity and injury characteristics of beet armyworm, Spodoptera exigua (Hübner), (Lepidoptera: Noctuidae) in Southern Region of Korea. Korean J. Appl. Entomol. 30(2): 124-129. | 미소장 |
21 | Tannic Acid, Protein, and Digestible Carbohydrate: Dietary Imbalance and Nutritional Compensation in Locusts ![]() |
미소장 |
22 | Raymond, M. 1985. Presentation d'un programme d'analyse log-probit pour micro-ordinnateur. Cah. ORSTOM, Ser. Ent. Med. et parasitol. 22: 117-121. | 미소장 |
23 | Bacillus thuringiensis proteases: Production and role in growth, sporulation and synergism ![]() |
미소장 |
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