표제지
목 차
ABSTRACT 5
I. 서 론 8
II. 재료 및 방법 12
1. 공시재료 및 genomic DNA 분리 추출 12
2. PCR 반응조건 및 통계분석 13
III. 결과 및 고찰 15
IV. 요 약 20
Ⅴ. 인 용 문 헌 22
Fig. 1. Photo of Korean bullhead, Pseudobagrus fulvidraco 12
Fig. 2. Diagram is flow-chart of PCR 14
Fig. 3. Amplification products were electrophoresed on a 1.4% agarose gel with TBE (0.09 M Tris, pH 8.5; 0.09 M boric acid; 2.5 mM EDTA) and detected by staining with ethidium bromide. Individual specific RAPD patterns of cultured Korean bullhead (Pseudo 30
Fig. 4. PCR-RAPD products of cultured Korean bullhead from an aquaculture facility amplified by arbitrary OPA-04 (AATCGGGCTG). Each lane (1∼10) shows different individual 31
Fig. 5. Individual specific RAPD patterns in cultured Korean bullhead from an aquaculture facility amplified by arbitrary OPA-08 (GTGACGTAGG). Each lane (1∼11) shows different individual 32
Fig. 6. Specific RAPD patterns of cultured Korean bullhead from an aquaculture facility amplified by arbitrary primer OPA-11 (CAATCGCCGT). Each lane (1∼11) shows different individual 33
Fig. 7. RAPD profiles in cultured Korean bullhead from an aquaculture facility amplified by primer OPA-16 (AGCCAGCGAA). Each lane 34
Fig. 8. Individual specific RAPD patterns amplified by arbitrary OPA-19 (CAAACGTCGG) in cultured Korean bullhead. Each lane (1∼11) 35
Fig. 9. Polymorphic and specific RAPD bands generated in cultured and wild Korean bullhead populations by arbitrary primer OPA-16 (AGCCAGCGAA). Each lane (1∼22) shows different individual 36
Table 1. Primers and primer sequences used for the detection of polymorphism in cultured Korean bullhead (Pseudobagrus fulvidraco) 26
Table 2. Number of RAPD bands for single primer using agarose gel in cul tured Korean bullhead (Pseudobagrus fulvidraco) from an aquaculture from Dangjin gun 27
Table 3. Bandsharing (BS) value within the cultured Korean bullhead (Pseudobagrus fulvidraco) population from an aquaculture facility (mean or mean�S.E) 28
Table 4. Comparisons of the RAPD profiles of cultured and wild Korean b ullhead populations from Dangjin gun(lanes 1∼11 from an aquacul ture facilities, lanes 12∼22 from a natural lake) obtained with arbit rary primer OPA-16 (AG 29