표제지
목차
Ⅰ. 서론 7
Ⅱ. 재료 및 방법 12
1. 공시동물 12
2. Sample 채취 및 Genomic DNA 추출 12
3. Primer의 제작 12
4. PCR 증폭 14
5. Enzyme 처리 15
6. 통계처리 15
Ⅲ. 결과 및 고찰 17
1. Genomic DNA의 추출 및 확인 17
2. PCR 증폭 산물의 확인 18
3. PSS 유전자형 및 유전자의 출현율 19
Ⅳ. 요약 26
Ⅴ. 참고문헌 28
ABSTRACT 33
감사의 글 35
Table 1. The average distribution of PSS genotypes determined by PCR-RFLP in pigs, and chi-square test for goodness of fitness by Hardy-Weinberg equilibrium 20
Table 2. The distribution of PSS genotypes determined by PCR-RFLP in Cheongyang and Boryeong area, and chi-square test for goodness of fitness by Hardy-Weinberg equilibrium 23
Table 3. The frequencies of PSS genes in Cheongyang and Boryeong area 24
Fig. 1. Partial sequence of ryanodine receptor(RYR1) gene. The site of sence primer and antisence primer sequence is underlined, and the point mutation site is shown by an vertical arrow. 13
Fig. 2. Diagram is flow-chart of PCR. 16
Fig. 3. The length of 20kb and over from hair follicles genomic DNA of pigs. 17
Fig. 4. PCR products of 1881bp fragment including mutation point in ryanodine receptor(M: Molecular size standard (100 bp DNA ladder plus, lane 1∼5: PCR products). 18
Fig. 5. Band patterns of different genotypes in 1.0% agarose gel following digestion of PCR products with Cfo I restriction enzyme. The normal genotype(N/N) shows two digested fragments(1250bp, 631bp), heterozygous genotype(N/n) shows two digested fragments and one undigested fragments(1881bp, 1250bp, 631bp), and PSS genotype(n/n) shows only one undigested fragment(1881bp). 19