표제지
요약
목차
Ⅰ. 緖論 6
Ⅱ. 材料및 方法 8
1. 시료의 제조 8
2. B16F10 melanoma cell의 배양 8
3. 세포내 melanin의 농도 측정 9
4. 세포외 melanin의 분비 측정 9
5. 세포의 형태적 관찰 및 증식 측정 9
6. 세포내 tyrosinase의 활성 측정 10
7. SDS-PAGE 및 Western blotting 10
Ⅲ. 實驗結果 11
1. 白附子와 白芷가 세포내 melanin생성에 미치는 영향 11
2. 白附子와 白芷가 세포외 melanin분비에 미치는 영향 14
3. 白附子와 白芷가 세포의 형태와 증식에 미치는 영향 17
4. 白附子와 白芷가 tyrosinase의 활성에 미치는 영향 23
5. 白附子와 白芷가 tyrosinase,TRP-1및 TRP-2의 발현에 미치는 영향 25
Ⅳ. 考察 27
Ⅴ. 結論 35
參考文獻 36
Abstract 41
Fig. 1. Effects of AEAK (A) and AEAD (B) on melanin contents in α-MSH-treated B16F10 cell 12
Fig. 2. Effects of AEAK (A) and AEAD (B) on melanin contents in α-MSH-treated B16F10 cells 13
Fig. 3. Effects of AEAK (A) and AEAD (B) on extracellular melanin accumulation in α-MSH-treated B16F10 cells 15
Fig. 4. Effects of AEAK (A) and AEAD (B) on extracellular melanin accumulation in α-MSH-treated B16F10 cells 16
Fig. 5. Effects of AEAK (A) and AEAD (B)on morphological chances in α-MSH-treated B16F10 cells 19
Fig. 6. Effects of AEAK (A) and AEAD (B)on morphological chances in α-MSH-treated B16F10 cells 20
Fig. 7. Effects of AEAK (A) and AEAD (B)on cellproliferation in α-MSH-treated B16F10 cells 21
Fig. 8. Effects of AEAK (A) and AEAD (B)on apoptotic cell death in α-MSH-treated B16F10 cells 22
Fig. 9. Effects of AEAK (A) and AEAD (B) on tyrosinase activity in α-MSH-treated B16F10 cells 24
Fig. 10. Effects of AEAK (A) and AEAD (B) on the protein levels of tyrosinase, TRP-1, and TRP-2 in α-MSH-treated B16F10 cells 26