국문목차
표제지=0,1,4
감사의 글=I,5,1
목차=II,6,3
LIST OF TABLES=V,9,1
LIST OF FIGURES=VI,10,1
ABSTRACT=VII,11,2
I. 서론=1,13,4
II. 재료 및 방법=5,17,1
1. 실험재료=5,17,1
1) 장소선정 및 시료채취=5,17,2
2) 표준균주=7,19,1
3) 시약 및 사용배지=7,19,3
4) 사용기기=10,22,1
2. 실험방법=10,22,1
1) 시료의 전처리=10,22,2
2) 위생지표세균의 측정=11,23,2
3) 공중 부유균 측정=13,25,1
4) 병원성 미생물 검사=13,25,2
(1) Escherichia coli 0157:H7=14,26,2
(2) Salmonella spp=15,27,2
(3) Listeria monocytogenes=16,28,1
(4) Staphylococcus aureus=16,28,3
5) Statpplococcus aureus에 대한 enterotoxin 생성 gene검색=19,31,1
(1) Genomic DAN 추출=19,31,1
(2) Oligonocleotide primer=20,32,1
(3) 반응 조건=20,32,1
① PCR mixture 조건=20,32,1
② PCR 온도 조건=20,32,2
III. 결과 및 고찰=22,34,1
1. 위생지표세균의 분석=22,34,1
1) 제조 기구=22,34,2
2) 제조기기=24,36,1
3) 제조 종사자=25,37,1
4) 원료와 토마토쥬스=26,38,2
2. 공중 부유균 검색=28,40,1
3. 병원성미생물의 분석=29,41,1
1) E. coli O157 H:7=29,41,2
2) Salmonella spp=30,42,2
3) Listeria monocytogenes=31,43,1
4) Staphplococcus aureus=32,44,2
5) 분리된 Staphylococcus aureus의 enterotoxin gene과 tsst gene 검색=34,46,1
(1) SEA gene 검색=35,47,1
(2) SEB gene 검색=36,48,1
(3) SEC gene 검색=37,49,1
(4) SED gene 검색=38,50,1
(5) SEE gene 검색=39,51,1
(6) TSST gene 검색=40,52,1
6) Enterotoxin gene 검색에 대한 종합 평가=41,53,2
IV. 적요=43,55,2
V/(VI). 참고문헌=45,57,7
Table1. Sample sources for microbiological assessment=6,18,1
Table2. The media and 3M petrifilm used for the measurement of sanitary indication bacteria air-borne microorganisms=8,20,1
Table3. The media and reagents used for the isolation of pathogenic bacteria=9,21,1
Table4. Biochemical characteristics of pathogenic bacteria=18,30,1
Table5. Primers used for amplification of Staphylococcal enterotoxin ene and tsst ene=21,33,1
Table6. Thermal condition for amplification of Staphylococcal enterotoxin gene and tsst gene=21,33,1
Table7. Estimation of sanitary indication bacteria on utensils=23,35,1
Table8. Estimation of sanitary indication bacteria on machinery=24,36,1
Table9. Estimation of sanitary indication bacteria on employees=25,37,1
Table10. Estimation of sanitary indication bacteria on raw material and tomato juice=27,39,1
Table11. Estimation of air-borne microorganisms in shops=28,40,1
Table12. Estimation of sanitary pathogen bacteria in 5 shops=29,41,1
Table13. The division of detected Staphylococcus aureus in 5 shops in accordance with the items=33,45,1
Table14. Screening of Staphylococcal enterotoxin genes in the tomato juice shop=42,54,1
Fig.1. The composition of HACCP system=3,15,1
Fig.2. Procedure for estimation of aerobic plate count and coliform group=12,24,1
Fig.3. Biochemical test procedures for the isolation of pathogenie bacteria=14,26,1
Fig.4. PCR result for SEA gene from detected S. aureus in tomato juice shops=35,47,1
Fig.5. PCR result for SEB gene from detected S. aureus in tomato juice shops=36,48,1
Fig.6. PCR result of SEC gene from detected S. aureus in tomato juice shops=37,49,1
Fig.7. PCR result of SED gene from detected S. aureus in tomato juice shops=38,50,1
Fig.8. PCR result of SEE gene from detected S. aureus in tomato juice shops=39,51,1
Fig.9. PCR result of TSST gene from detected S. aureus in tomato juice shops=40,52,1