표제지
목차
I. 서론 7
II. 재료 및 방법 10
1. 재료 10
2. 게놈 DNA 추출과 RAPD 분석 10
3. 데이터 분석 11
4. 군집 및 주성분 분석 13
III. 결과 14
IV. 고찰 22
참고문헌 26
영문요약 30
Appendix 1. RAPD data set at nine populations in Korea Population = PAN 32
Appendix 2. Measures of genetic variation for RAPD generated among populations for all loci 41
Appendix 3. Esitimates of genetic diversity of A. sessiliflorus in Korea for all loci except monomorphic loci 44
Table 1. List of decamer oligonucleotide utilized as RAPD primers, their sequences, and associated polymorphic fragments amplified in the Acanthopanax sessiliflorus. 12
Table 2. Measures of genetic variation for RAPD generated among populations. The number of polymorphic loci (PA), percentage of polymorphism (Pp), mean number ofalleles per locus (A), effective number of alleles per locus (AE), gene diversity (H), and ph 0
Figure 1. Collection sites of Acanthopanax sessiliflorus for RAPD analysis 9
Figure 2. RAPD profiles produced by OPE12 primer in A sessiliflorus with a molecular size marker on the left. M: molecular size marker. GIR, PAN, and ODA are the same as Figure1. 16
Figure 3. A dendrogram for A. sessiliflorus populations based on RAPD analysis 20
Figure 4. Associations among ten populations of A. sessiliflorus revealed by principal coordinate analysis estimated from RAPD data of twenty-one primer combinations. Wild and cultivated populations are designated by squares and circles, respectivel. 21