표제지
목차
I. 서론 9
II. 연구사 12
1. 핵형분석 12
2. 염색체의 구조와 Banding 13
1) GTG-banding 13
2) C-banding 14
3) AgNORs 16
3. 말의 핵형 양상 18
III. 재료 및 방법 28
1. 공시동물 28
2. 혈액배양을 이용한 염색체 분리 28
1) 혈액 세포배양 28
2) 중기상 유도 29
3) 저장처리 29
4) 고정처리 29
5) 표본제작 30
3. Banding 31
1) GTG-banding 31
2) C-banding 32
3) AgNOR staining 32
4. 통계분석 33
IV. 결과 및 고찰 34
1. 제주마 염색체 수 및 형태적 특징 34
2. 제주마 염색체의 G-banding 양상 40
3. 제주마 염색체의 C-banding 양상 54
4. 제주마 염색체의 NORs 59
V. 결론 64
VI. 참고문헌 66
ABSTRACT 83
感謝의 글 85
Table 1. Number of chromosome of the Equus genus 19
Table 2. International system for cytogenetic nomenclature of the domestic horse 21
Table 3. Components of complete media for chromosome preparation using blood lymphocytes 29
Table 4. The centromeric index and relative length of Jeju horse chrornosomes 35
Table 5. The centromeric index and relative length of Thoroughbred horse chromosomes 38
Table 6. Landmarks and description of G-banded chromosomes of Jeju horse 49
Table 7. The types and frequencies of C-band heteromorphism on the horse chromosomes 8 58
Table 8. Number of AgNORs on chromosomes 1, 26 and 31 in horse 61
Table 9. Number of AgNORs on chromosomes in male and female Jeju horse 62
Fig. 1. The current standard karyotype for the horse as defined by the International system for cytogenetic nomenclature of the domestic horse(ISCNH 1997) 27
Fig. 2. Diagram of chromosome preparation from whole blood culture 31
Fig. 3. Idiogram of relative length and centromere position of the horse chromosome The herizontal bars indicate the centromere position. * The relative length of chromosomes in Jeju horse and Thoroughbred horse is 39
Fig. 4. G-banded metaphase spread of Jeju horse; (a) male Fig. 4. G-banded metaphase spread of Jeju horse; (a) male (2n=64, XY (b) female (2n=64, XX) 43
Fig. 5. G-banded karyotype of Jeju horse male 44
Fig. 6. G-banded karyotype of Jeju horse female 45
Fig. 7. G-banded metaphase spread of Thoroughbred horse; (a) male(2n=64, XY) (b) female (2n=64, XX) 46
Fig. 8. G-banded metaphase karyotype of Thoroughbred horse male. 47
Fig. 9. G-banded karyotype of Thoroughbred horse female 48
Fig. 10. Idiogrammatic representation of G-banded chromosomes of Jeju horse ,male (2n=64, XY) 53
Fig. 11. Metaphase spread of C- banded chromosomes of the Jeju horse female 56
Fig. 12. The C- banded karyotype of the Jeju horse female 57
Fig. 13. C-band heteromorphic pattern on Jeju horse chromosomes 8 58
Fig. 14. AgNORs pattern on metaphase chromosomes of Jeju horse 59
Fig. 15. The karyotype of Jeju horse after AgNOR staining 60