표제지
목차
Abstracts 10
I. 서론 13
II. 재료 및 방법 15
1. 실험 재료 15
2. 실험 방법 15
1) 화학성분 15
(1) 일반성분 분석 15
(2) 무기성분 분석 15
(3) 유리당 분석 16
(4) 구성아미노산 분석 16
(5) 유리아미노산 분석 17
(6) 총 플라보노이드 화합물 분석 17
(7) 총 페놀 화합물 분석 17
(8) 비타민 C 분석 17
2) 생리적 기능성 18
(1) 추출방법 18
(2) DPPH radical 소거 활성 18
(3) 환원력 19
(4) 자동산화 억제력 19
(5) Tyrosinase 저해 활성 19
(6) 아질산염 소거 활성 20
(7) 인체 암세포주에 대한 세포독성 20
III. 결과 및 고찰 23
1. 화학성분 23
1) 일반성분 23
2) 무기성분 24
3) 유리당 25
4) 구성아미노산 25
5) 유리아미노산 26
6) 총 플라보노이드 화합물 함량 29
7) 총 페놀 화합물 함량 30
8) 비타민 C 함량 31
2. 생리적 기능성 32
1) DPPH radical 소거 활성 32
2) 환원력 35
3) 자동산화 억제력 36
4) Tyrosinase 저해 활성 39
5) 아질산염 소거 활성 40
6) 항암 활성 42
IV. 적요 44
V. 참고문헌 46
감사의 글
Table 1. Proximate compositions of white and red lotus 23
Table 2. Minerals content of white and red lotus 24
Table 3. Free sugars content of white and red lotus 25
Table 4. Total amino acids content of white and red lotus 27
Table 5. Free amino acids content of white and red lotus 28
Fig. 1. Photograph of white and red lotus. 15
Fig. 2. Procedure of cytotoxicity assay against human tumor cell in vitro by SRB method. 22
Fig. 3. Total flavonoids content of white and red lotus. 29
Fig. 4. Total phenol compounds content of white and red lotus. 30
Fig. 5. Ascorbic acid content of white and red lotus. 31
Fig. 6. DPPH radical scavenging activities of various solvent fractions from ethanol extract of white lotus leaf. 33
Fig. 7. DPPH radical scavenging activities of various solvent fractions from ethanol extract of white lotus root. 33
Fig. 8. DPPH radical scavenging activities of various solvent fractions from ethanol extract of red lotus leaf. 34
Fig. 9. DPPH radical scavenging activities of various solvent fractions from ethanol extract of red lotus root. 34
Fig. 10. Reducing power of various solvent fractions from ethanol extract of white and red lotus. 35
Fig. 11. Antioxidative activities of various solvent fractions from ethanol extract of white lotus leaf on linoleic acid at 1mg/mL. 37
Fig. 12. Antioxidative activities of various solvent fractions from ethanol extract of white lotus root on linoleic acid at 1mg/mL 37
Fig. 13. Antioxidative activities of various solvent fractions from ethanol extract of red lotus leaf on linoleic acid at 1mg/mL. 38
Fig. 14. Antioxidative activities of various solvent fractions from ethanol extract of red lotus root on linoleic acid at 1mg/mL. 38
Fig. 15. Inhibitory effects of various solvent fractions from ethanol extract of white and red lotus on mushroom tyrosinase. 40
Fig. 16. Nitrite scavenging activities of various solvent fractions from ethanol extract of white and red lotus. 41
Fig. 17. Cytotoxicity of various solvent fractions from ethanol extract of white and red lotus against human lung cancer cell line. 43
Fig. 18. Cytotoxicity of various solvent fractions from ethanol extract of white and red lotus against human colon cancer cell line. 43