표제지
목차
I. 서론 9
II. 연구사 11
1. 면역 작용 기전 및 사이토카인 11
2. 영양소 및 에너지 수준이 면역 작용에 미치는 영향 19
III. 재료 및 방법 26
1. 시험동물 및 시험사료 26
1) 시험동물 및 시험사료 26
2) 시험 사료 및 관리 28
2. 시험방법 28
1) 시료채취 28
2) 혈액학적 검사 28
3) Real-Time PCR (Real-time Ploymerase Chain Reaction)을 이용한 mRNA 발현 29
(1) Total RNA 추출 29
(2) Reverse Transcription 30
(3) Primer 제작 31
(4) Real-time PCR 31
4) Western blot 32
(1) Total protein 추출 32
(2) Western blot 32
(3) Coomassie blue 염색 33
5) 혈액내 IgG 분석 36
3. 통계처리 36
IV. 결과 및 고찰 37
1. 제한급여에 따른 사료 섭취량, 체중, 증체량, 사료 요구율 37
2. 제한 급여에 따른 장기무게 40
3. 제한급여에 따른 혈액 중 생화학 성분 분석 43
4. 혈액내 혈구 구성, 백혈구 총 개수 및 백혈구 감별계수 46
5. 조직내 TNF-α, iNOS 및 IL-4의 mRNA 및 단백질 발현 54
V. 결론 61
VI. 참고문헌 64
ABSTRACT 76
감사의 글 79
Table 1. Formula of purified diets fed to SD rats 27
Table 2. Sequences of primers used in Real-time PCR 31
Table 3. Effect of the stress induced by the restriction of energy and feed intake on growth performance in SD rats 39
Table 4. Effect of the stress induced by the restriction of energy and feed intake on weights of the spleen, liver, thymus and brain in SD rats 41
Table 5. Effect of the stress induced by energy restriction and feed intake restriction on blood biochemical profiles in SD rats 45
Table 6. Effect of the stress induced by the restriction of energy and feed intake on the profiles of blood cells in SD rats 48
Table 7. Effect of the stress induced by the restriction of energy and feed intake on white blood cell(WBC) differential count in SD rats 49
Table 8. Effect of the stress induced by the restriction of energy and feed intake on differential count of white blood cell in SD rats 51
Table 9. Effect of the stress induced by the restriction of energy and feed intake on the level of blood IgG in SD rats 53
Table 10. Effect of the stress induced by the restriction of energy and feed intake on the mRNA expression of TNF-α, iNOS and IL-4 in SD rats 58
Table 11. Effect of the stress induced by the restriction of energy and feed intake on protein expression of TNF-α, iNOS and IL-4 in SD rats 59
Fig. 1. Diagram of Cellular and humoral immunity (A, cited from 정, 2007) and T-lymphocyte development and its function (B, cited from Jolly, 2004). 18
Fig. 2. Coomassie blue staining of thymic protein in SD rats. 35
Fig. 3. Effect of the stress induced by the restriction of energy and feed intake on weight of the liver, spleen, thymus and brain in SD rats. 42
Fig. 4. Effect of the stress induced by the restriction of energy and feed intake on white blood cell differential count in SD rats(A: Lympocyte, B: Neutrophil, C: Monocyte, D: Eosinophil, E: Basophil). 50
Fig. 5. Effect of the stress induced by the restriction of energy and feed intake on protein expression of IL-4(A), iNOS(B) and TNF-α(C) in SD rats. 60