표제지
목차
기호설명 8
Ⅰ. 서론 9
Ⅱ. 재료 및 방법 12
1. 실험재료 및 기기 12
2. Propionibacterium acnes 배양 12
3. 뽕잎 추출물의 농도별 동충하초 균사체 배양액 제조 13
4. 뽕잎 추출물에 동충하초 균사체 배양일수별 배양액 제조 13
5. 배양일수별 단백질 패턴 확인 14
6. 디스크법(Disk Diffusion test)에 의한 항여드름균 활성 측정 14
7. 배지별 동충하초 균사체 배양액 제조 14
8. 뽕잎 추출물에 동충하초 균사체 배양액의 열 안정성 평가 15
9. 뽕잎 추출물에 동충하초 균사체 배양액의 유기 용매에 의한 여드름균 생육 억제 활성 물질 분리 15
Ⅲ. 결과 및 고찰 17
1. 뽕잎의 농도별 여드름균 항균 활성 측정 17
2. 동충하초 균사체의 배양 날짜별 여드름균 항균 활성 측정 20
3. 배양일수별 단백질 패턴 확인 23
4. 배지별 동충하초 균사체의 항 여드름균 활성 비교 24
5. 뽕잎추출물에 동충하초 균사체 배양액의 농도별 여드름균 생육억제 활성 측정 27
6. 뽕잎 추출물에 동충하초 균사체 배양액의 열 안정성 조사 28
7. 뽕잎 추출물에 동충하초 균사체 배양액의 유기 용매에 의한 여드름균 생육 억제 활성 물질 분리 30
Ⅳ. 결론 33
참고문헌 34
Abstract 37
감사의 글 39
Fig. 1. Anatomy of the skin. 11
Fig. 2. Organic solvent extraction condition of mycelial culture broth of P. japonica in the 3% Mulberry leaf extract. 16
Fig. 3. Growth inhibition of P. acnes by treatment of mycelial culture broth of P. japonica in each concentration of Mulberry leaf extract for 15 days. 18
Fig. 4. Growth inhibition of P. acnes by treatment of mycelial culture broth of P. japonica in each concentration of Mulberry leaf extract for 15 days. 19
Fig. 5. Growth inhibition of P. acnes by treatment of the time-course mycelial culture broth of P. japonica in 3% Mulberry leaf extract. 21
Fig. 6. Growth inhibition of P. acnes by treatment of the time-course mycelial culture broth of P. japonica in 3% Mulberry leaf extract. 22
Fig. 7. Protein patterns in the time-course mycelial culture broth of P. japonica in 3% Mulberry leaf extract. 23
Fig. 8. Growth inhibition of P. acnes by treatment of the mycelial culture broth of P. japonica in each media for 25days. 25
Fig. 9. Growth inhibition of P. acnes by treatment of the mycelial culture broth of P. japonica in each media for 25days. 26
Fig. 10. Growth inhibition of P. acnes according to treatment concentration of mycelial clture broth of P. japonica in 3% Mulberry leaf extract for 25days. 27
Fig. 11. Growth inhibition of P. acnes according to treatment concentration of mycelial clture broth of P. japonica in 3% Mulberry leaf extract for 25days. 28
Fig. 12. Growth inhibition of P. acnes after heating at 100℃ for 15, 30, 45, 60, 90, and 120 min of mycelial culture broth of P. japonica in 3% Mulberry leaf extract for 25days. 29
Fig. 13. Growth inhibition of P. acnes by organic solvent extract of mycelial culture broth of P. japonica in the 3% Mulberry leaf extract. 31
Fig. 14. Growth inhibition of P. acnes by organic solvent extract of mycelial culture broth of P. japonica in the 3% Mulberry leaf extract. 32