표제지
목차
Ⅰ. 서론 7
Ⅱ. 재료 및 방법 11
1. 재료 11
2. Genomic DNA 추출 11
3. RAPD 분석 12
4. 데이터 분석 15
5. 군집 분석 16
Ⅲ. 결과 17
Ⅳ. 고찰 26
V. 결론 29
참고문헌 30
Appendix 34
Abstract 37
Table 1. Distribution of selected species of genus Malus in this study and their chromosome numbers 10
Table 2. List of decamer oligonucleotides utilized as primers, their sequences, and associated polymorphic fragments amplified in Malus taxa 14
Table 3. Measures of genetic variation for genus Malus. The number of polymorphic loci (Np), percentage of polymorphism (Pp), mean number of alleles per locus (A), effective number of alleles per locus (AE), gene diversity (H), and Shannon's 18
Table 4. Estimates of genetic diversity of Malus taxa. Total genetic diversity (HT), genetic diversity within populations (HS) proportion of total genetic diversity partitioned among populations (GST), and gene flow (Nm) 19
Table 5. Partitioning of the genetic diversity into within and among Malus taxa by RAPD 20
Table 6. Genetic identity (upper diagonal) of Malus taxa and genetic distances (low diagonal) based on RAPD analysis 23
Figure 1. RAPD profiles by OPD-05 for nine Malus taxa. 24
Figure 2. A phenogram showing the relationships among nine Malus taxa and two outgroups based on data of genetic distance obtained by RAPD. 25