표제지
목차
Abbreviations 8
I. 서론 9
II. 재료 및 방법 18
1. 식물재료 18
2. 발현 벡터 19
3. 형질전환 및 식물체 재분화 19
4. Genomic DNA 분리 24
5. Polymerase chain reaction (PCR) 분석 24
6. Total RNA 분리 및 Northern blot hybridization 25
7. Protein 분리 및 Fluorometric GUS assay 27
8. Histochemical staining for GUS activity 28
III. 결과 및 고찰 29
1. 형질전환 감자의 선발 효율 29
2. 형질전환체의 분자생물학적 확인 34
3. ibAGP1 프로모터와 ibAGP1 transit peptide가 형질전환 감자의 조직 내 GUS 단백질의 축적에 미치는 영향 38
4. Sucrose농도가 ibAGP1 프로모터 및 ibAGP1 transit peptide의 활성에 미치는 영향 47
IV. 결론 54
참고문헌 57
Abstract 71
Table 1. Composition of growth medium I 18
Table 2. Composition of growth medium II 21
Table 3. Effect of ibAGP1 promoter on the frequency of... 33
Fig. 1. Sucrose breakdown in the cytosol and starch synthesis... 14
Fig. 2. The schematic diagram of recombinant T-DNA... 20
Fig. 3. Schematic diagram of the potato transformation... 23
Fig. 4. Representative plant regeneration steps from potato leaf... 31
Fig. 5. PCR amplification of GUS gene fragment from... 35
Fig. 6. Northern blot hybridization of transgenic plants... 37
Fig. 7. The activities of ibAGP1 promoter and its transit... 39
Fig. 8. Detection of accumulated GUS enzyme in the leaf... 41
Fig. 9. The activities of ibAGP1 promoter and its transit... 43
Fig. 10. The effect of exogenous sucrose concentration on... 50
Fig. 11. Histochemical analysis of ibAGP1 promoter and... 53