표제지
목차
I. 서론 8
II. 재료 및 방법 13
1. 실험 동물 13
2. Genomic DNA 분리 및 정량 19
1) Genomic DNA 분리 19
2) Genomic DNA 정량 19
3. 경제형질 후보 유전자 Primer 합성 및 PCR-RFLP 분석 20
4. MS marker 합성 및 PCR 분석 23
1) MS marker 합성 23
2) PCR 분석 23
5.통계 분석 27
III. 결과 및 고찰 28
1. 유전자형 분석결과 28
2. MS marker 분석결과 34
IV. 참고문헌 51
Abstract 56
감사의 글 58
Table 1. Animal and data collection 14
Table 2. The primer design for PCR 21
Table 3. PCR reagent and thermal condition for each gene test 22
Table 4. Microsatellite marker names, primer oligonucleotide sequences 25
Table 5. PCR reagent and thermal condition for each MS test 26
Table 6. The association between MYF3 genotypes and economic traits in pig 32
Table 7. The association between GYS1 genotypes and economic traits in pig 33
Table 8. Analysis of PCR products by genotyper 35
Table 9. The association between S0355 alleles and economic traits in pig 43
Table 10. The association between SW1632 alleles and economic traits in pig 44
Table 11. The association between SW2185 alleles and economic traits in pig 45
Table 12. The association between SW256 alleles and economic traits in pig 46
Table 13. The association between SW 310 alleles and economic traits in pig 47
Table 14. The association between SW353 alleles and economic traits in pig 48
Table 15. The association between SW864 alleles and economic traits in pig 49
Table 16. The association between SW919 alleles and economic traits in pig 50
Figure 1. PCR-RFLP analysis image results of (A) LEP, (B) GYS1, (C)MYF3, (D) MYF5, (E) GHRH, (F) H-FABP (G) MYOG S; 100bp ladder size marker, P; PCR product 31