표제지
목차
I. 서론 9
II. 재료 및 방법 11
1. 공시균주 및 접종원 11
2. 배지제조 13
3. 배지성분 분석 14
4. 배양 및 생육특성 조사 14
III. 결과 및 고찰 15
1. 배지 성분분석 15
2. 폐배지 추출물의 저해능 시험 19
3. 배지별 배양조사 21
4. 배지별 버섯 발이조사 29
5. 배지별 생육조사 32
6. 폐배지 사용시 기대효과 43
IV. 결론 45
참고문헌 47
Abstract 49
감사의 글 51
Table 1. Chemical composition (DM basis) of mushroom culture medium 16
Table 2. Growth of mycelium on medium containing spent mushroom substrate (SMS) 22
Table 3. The particle size and mushroom yield on medium containing spent mushroom substrate (SMS) 34
Fig. 1. The pH of spent mushroom substrate (SMS) after fermented with yeast and Lactobacillus. 17
Fig. 2. The pH of medium containing sterilized spent mushroom substrate (SMS). 18
Fig. 3. Growth of mycelium on medium containing 10% spent mushroom substrate. 23
Fig. 4. Growth of mycelium on medium containing 20% spent mushroom substrate. 24
Fig. 5. Growth of mycelium on medium containing 30% spent mushroom substrate. 25
Fig. 6. The primordial development on medium containing spent mushroom substrate (SMS) after remove of old mycelium. 30
Fig. 7. The primordial development on medium containing spent mushroom substrate (SMS) after remove of old mycelium. 33
Fig. 8. The mushroom on medium containing fermented spent mushroom substrate (SMS) with yeast. 35
Fig. 9. The mushroom on medium containing fermented spent mushroom substrate (SMS) with Lactobacillus. 36
Fig. 10. The mushroom on medium containing fermented spent mushroom substrate (SMS) without treatment. 37
Fig. 11. The mushroom on medium containing non-treated spent mushroom substrate (SMS). 38
Fig. 12. The comparison of production cost according to added spent mushroom substrate (SMS). 44
photo. 1. Growth of mycelium on medium from heat extract (25℃, 7day). 20
photo. 2. Growth of mycelium on medium containing spent mushroom substrate (SMS) after 10 days of cultivation. 26
photo. 3. Growth of mycelium on medium containing spent mushroom substrate (SMS) after 20 days of cultivation. 27
photo. 4. Growth of mycelium on medium containing spent mushroom substrate (SMS) after 30 days of cultivation. 28
photo. 5. The primordial development on medium containing spent mushroom substrate (SMS) after 9 days from remove of old mycelium. 31
photo. 6. The primordial development on medium containing spent mushroom substrate (SMS) after 11 days from remove of old mycelium. 39
photo. 7. The primordial development on medium containing spent mushroom substrate (SMS) after 13 days from remove of old mycelium. 40
photo. 8. The primordial development on medium containing spent mushroom substrate (SMS) after 15 days from remove of old mycelium. 41
photo. 9. The mushroom cultivated on medium containing spent mushroom substrate (SMS). 42