표제지
목차
I. 서론 10
II. 재료 및 방법 14
1. 공시균주 14
2. 액체종균 최적배양 조건 14
1) 종균배양 배지 14
2) 300ℓ 액체배양탱크 14
3) Air cleaning system 18
4) 최적배지 선발 20
5) 대두박 최적 배지조성 22
6) 균사생장 길이측정 22
7) 건조 균사체량 측정 22
8) pH 측정 23
3. MIDI에 의한 미생물 균주 측정 23
4. 액체종균 접종량이 배양 및 생육에 미치는 영향 24
1) 공시배지 24
2) 병배지 살균 24
3) 병배지 접종 및 배양 생육 24
III. 결과 및 고찰 25
1. 실험상의 균사배양 최적배지 25
1) 최적배지 선발 25
2) 대두박 배지 최적조건 탐색 26
3) 배양 온도가 균사 생장에 미치는 영향 31
4) pH가 균사생육에 미치는 영향 33
2. Air cleaning system이 균사생육에 미치는 영향 36
1) Air cleaning system 36
2) Filter의 형태에 따른 균사생육 영향 38
3. 액체 배양 탱크에서 균사의 최적 배양 조건 40
1) 질소원의 영향 40
2) 통기량에 따른 영향 42
3) 배양 시간에 따른 CO₂의 변화 44
4) 최적 배양조건에서 균체량 및 pH 측정 45
5) 현미경상에서 배양기간별 P. eryngii 변화 47
4. MIDI. 에 의한 액체종균 오염균 분석 48
5. 액체 배양한 종균의 접종량에 따른 균사생육 영향 50
1) 종균 접종량이 균사생육에 미치는 영향 50
2) 종균 접종량에 따른 오염율 조사 53
3) 자실체 생육에 미치는 영향 54
IV. 적요 59
V. 참고문헌 61
Abstract 66
감사의글 68
Table.1. The comparison of oil free type and oil injection type of air cleaning system 19
Table.2. The composition of various media for liquid spawn culture of Pleurotus eryngii 21
Table.3. Change of length and dry weight of mycelium of P. eryngii by different growth media 25
Table.4. The composition of liquid soybean meal medium for P. eryngii 26
Table.5. Inhibitor bacterial strains isolated from mycelium of P. eryngii by MIDI 48
Table.6. Effect of cultivation time and on inoculum rate on the mycelial colony diameter of P. eryngii 51
Table.7. Effect of cultivation time on inoculum rate on the pH of P. eryngii 51
Table.8. Comparison of loss ratio according to the inoculum rate of the liquid spawn of P. eryngii 53
Table.9. Growth characteristics according to the inoculum rate of P. eryngii 58
Fig.1. Liquid cultivation tank 16
Fig.2. Gastac GV-100 17
Fig.3. The comparison of oil free type and oil injection type of air cleaning system 18
Fig.4. Effect of Soybean meal concentration on the mycelial growth of P. eryngii for 15days and analysis of pH change 27
Fig.5. Effect of Soybean meal(0.3%) and Sugar concentration on the mycelial growth of P. eryngii and analysis of pH change 28
Fig.6. Effect of Soybean meal(0.3%), sugar(3%), and KH₂PO₄concentration on the mycelial growth of P. eryngii for 15days and analysis of pH change 29
Fig.7. Effect of Soybean meal(0.3%), Sugar(3%), KH₂PO₄(0.1%), MgSO₄·7H₂O concentration on the mycelial growth of P. eryngii for 15days and analysis of pH change 30
Fig.8. Effect of temperature on the mycelial growth and diameter of mycelium of P. eryngii after 15days 32
Fig.9. Effect of pH on the mycelial growth of P. eryngii 34
Fig.10. Effect of pH on the Erlenmeyer flask cultivation of P. eryngii after 15days 35
Fig.11. The comparison of oil free type and oil injection type of air cleaning system 37
Fig.12. The comparison of single filter and double filter in the 300ℓ liquid culture tank aeration on the mycelial growth of P. eryngii for 10days 39
Fig.13. Effect of the soybean meal concentration in the 300ℓ liquid culture tank on the mycelial growth of P. eryngii for 10days 41
Fig.14. Effect of the aeration in the 300ℓ liquid culture tank on the mycelia growth of P. eryngii after 10days 43
Fig.15. Effect of the CO₂ in the 300ℓ liquid culture tank on the mycelia growth of P. eryngii for 15days 44
Fig.16. The change of dry weight of mycelium of P. eryngii and pH of the liquid culture tank under the optimum condition for culture 46
Fig.17. The microphotograph of mycelia of P. eryngii 47
Fig.18. An analysis of diversity for bacteria causing cultivation barrier(MIDI) 49
Fig.19. Mycelial growth of P. eryngii according to the inoculum rate and cultivation time 52
Fig.20. New mycelium induction of P. eryngii in each different inoculum rate 55
Fig.21. Fruiting bady induction of P. eryngii in each different inoculum rate 56
Fig.22. Fruiting bady induction of P. eryngii in each different inoculum rate 57