title page
Contents
I. Introduction 6
II. Materials and methods 9
A. Materials 9
B. Cell culture 10
C. Cell viability assay 10
D. Morphological observation of nuclear change 11
E. Measurement of oligonucleosomal DNA fragmentation 11
F. Measurement of apoptosis in cells 11
G. Flow cytometric measurement of mitochondrial transmembrane potential 12
H. Measurement of Bax, Bcl-2 and cytochrome c levels 12
I. Measurement of caspase-3 activity 14
J. Measurement of intracellular ROS formation 15
K. Measurement of total glutathione 15
L. Statistical analysis 16
III. Results 17
A. Effect of tyrphostin AG126 on 7-ketocholesterol-induced cell death and nuclear damage 17
B. Effect of tyrphostin AG126 on 7-ketocholesterol-induced cytochrome c release and caspase-3 activation 23
C. Effect of tyrphostin AG126 on 7-ketocholesterol-induced formation of ROS and depletion of GSH 30
IV. Discussion 35
V. REFERENCES 39
- 국문초록 - 45
ABSTRACT 47
감사의 글 49