표제지
목차
ABSTRACT 8
Ⅰ. 서론 9
Ⅱ. 재료 및 방법 12
1. 실험재료 12
1) 샘플링장소 선정 12
2) 시료채취 12
3) 미생물 검사항목 및 사용배지 13
2. 실험방법 15
1) 시료의 전처리 15
2) 위생지표세균 측정 15
3) 병원성 미생물 검사 17
4) 공중낙하균 검사 18
5) 통계분석 18
Ⅲ. 결과 및 고찰 19
1. 딸기농장별 위생지표세균 오염도 19
1) 일반세균(Total bacteria) 19
2) 대장균군 (Coliforms) 20
3) 대장균(E. coli) 21
2. 딸기농장별 병원성미생물 오염도 22
1) E. coli O157 22
2) S. aureus 23
3) Salmonella 24
4) B. cereus 26
3. 딸기농장별 공중낙하균 검색 27
1) 위생지표세균 27
2) 병원성 미생물 28
4. 딸기농장별 농업용수의 오염도 29
1) 위생지표세균 29
2) 병원성 미생물 30
5. 딸기재배 시설의 미생물학적 위해요소 관리방안 31
Ⅳ. 적요 33
Ⅵ. 참고 문헌 35
Table 1. Collected samples and the number of samples for each sampling place 13
Table 2. The media used for the measurement of microorganisms 14
Table 3. Levels of E. coli of samples obtained from surroundings of strawberry farms 22
Table 4. Levels of E. coli O157 of samples obtained from surroundings of strawberry farms 23
Table 5. Levels of Salmonella of samples obtained from surroundings of strawberry farms 25
Table 6. Levels of B. cereus of samples obtained from surroundings of strawberry farms 26
Table 7. Levels of cell counts of airborne microorganisms such as E. coli O157, Salmonella, S. aureus and B. cereus in strawberry farms 30
Table 8. Sanitary management guidelines for APC 32
Fig. 1. Strawberry for microbial analysis on farms. 14
Fig. 2. Procedure for evaluation of total bacteria and coliform. 16
Fig. 3. Procedure for evaluation of E. coli and pathogenic bacteria. 17
Fig. 4. Levels of total bacteria of samples obtained from surroundings of strawberry farms. 19
Fig. 5. Levels of coliforms of samples obtained from surroundings of strawberry farms. 21
Fig. 6. Levels of S. aureus of samples obtained from surroundings of strawberry farms. 24
Fig. 7. Levels of cell counts of airborne microorganisms such as total bacteria, coliform, and E. coli in strawberry farms. 27
Fig. 8. Levels of cell counts of airborne microorganisms such as E. coli O157, Salmonella, S. aureus, and B. cereus in strawberry farms. 28
Fig. 9. Levels of cell counts of Water microorganisms such as total bacteria, coliform, and E. coli in strawberry farms. 29