표제지
목차
I. 서론 9
II. 연구사 11
1. 식물체의 화학성분과 항산화 작용 11
2. 가시오갈피 19
3. 두충 22
III. 재료 및 방법 27
1. 시험재료 및 사양관리 27
1) 공시동물 및 시험설계 27
2) 사료준비 및 사양관리 27
2. 시험방법 31
1) 시료채취 및 분석방법 31
2) 체중 및 사료섭취량 32
3) 난질 분석 32
4) 항산화 활성도 분석 32
5) 양적텔로미어 함량분석 34
6) 통계처리 36
IV. 결과 및 고찰 37
1. 체중 및 증체량 37
2. 난질분석 39
3. 소장 점막세포내 및 간 조직내 항산화 효소분석 42
4. 텔로미어 상대적 함량 분석 48
V. 요약 53
VI. 참고문헌 55
ABSTRACT 70
감사의 글 72
Table 1. Major phytochemicals and their functions 18
Table 2. Experimental design for the dietary supplementation of Acanthopanax senticosus leaf and Eucommia ulmoides leaf on laying hens 29
Table 3. Chemical composition of Acanthopanax. senticosus leaf and Eucommia ulmoides leaf 30
Table 4. Chemical composition of experimental diets fed to laying hens 30
Table 5. Effects of dietary supplementation of Acanthopanax senticosus leaf and Eucommia ulmoides leaf on body weight and gain from 20 wks to 30 wks laying hens 38
Table 6. Effects of dietary supplementation of Acanthopanax senticosus leaf and Eucommia ulmoides leaf on egg quality in 30 wks laying hens 41
Table 7. Effects of dietary supplementation of Acanthopanax senticosus leaf and Eucommia ulmoides leaf on the activities of SOD, GST and GSH-Px and the level of MDA in the small intestine from 30 wks laying hens 46
Table 8. Effects of dietary supplementation of Acanthopanax senticosus leaf and Eucommia ulmoides leaf on the activities of SOD, GST and GSH-Px and the level of MDA in the liver from 30 wks laying hens 47
Table 9. Effects of dietary supplementation of Acanthopanax senticosus leaf and Eucommia ulmoides leaf on the amount of telomeric DNA in 30 wks laying hens 51
Fig 1. The representative telomere distribution patterns of interphase nuclei of lymphocytes in layer by FISH using telomeric DNA probe; ATD(Amount of Telomeric DNA) 52