표제지
감사의 글
목차
ABSTRACT 9
Ⅰ. 서론 10
Ⅱ. 재료 및 방법 13
1. 실험재료 13
1) 사료종류 13
2) 방사선조사시설 13
3) 검사균주 및 분석방법 14
4) 시약 및 사용배지 15
2. 실험방법 16
1) 시료의 방사선 조사 16
2) 위생지표세균 측정 17
3) 병원성 미생물 검사 19
Ⅲ. 결과 및 고찰 22
1. 위생지표세균 오염도 22
1) 일반세균 22
2) 대장균군 24
3) 대장균 26
2. 병원성미생물 오염도 28
1) E. coli O157 28
2) L. monocytogenes 30
3) Salmonella spp. 32
4) S. aureus 34
5) B. cereus 36
Ⅳ. 적요 38
Ⅵ. 참고 문헌 39
Table 1. Microorganisms and methods for microbiological analysis 14
Table 2. The media and identification kits used for the microbiological analysis. 15
Table 3. Detection of E. coli from feeds treated by radiation 27
Table 4. Detection of E. coli O157 from feeds treated by radiation 29
Table 5. Detection of L. monocytogenes from feeds treated by radiation 31
Table 6. Detection of Salmonella spp. from feeds treated by radiation 33
Table 7. Detection of S. aureus from feeds treated by radiation 35
Table 8. Detection of B. cereus from feeds treated by radiation 37
Fig. 1. Principles of gamma irradiation 11
Fig. 2. Gamma irradiation facility(A) and cobalt-60 source(B) 13
Fig. 3. Gamma irradiation of feeds 16
Fig. 4. Procedure for the evaluation of total bacteria and coliform 17
Fig. 5. Procedure for the determination of E. coli 18
Fig. 6. Procedure for the determination of pathogenic bacteria 19
Fig. 7. Procedure for the evaluation of S. aureus and B. cereus 21
Fig. 8. Changes in total bacteria counts from feeds treated by radiation 23
Fig. 9. Changes in coliform counts from feeds treated by radiation 25