표제지
목차
Ⅰ. 緖言 8
Ⅱ. 硏究史 10
1. 미생물을 이용한 선충 방제 10
2. 선충포식성곰팡이의 특성 11
3. 식물기생성 선충 14
4. 선충 방제 방법 16
Ⅲ. 材料 및 方法 18
1. 공시 균주 18
2. M. thaumasium WHJ8의 배양 조사 18
1) 최적 액체배지 선발 및 배양 조건 탐색 18
2) 최적 고체배지 선발 및 배양 조건 탐색 19
3. M. thaumasium WHJ8의 제제화 및 대량 배양 19
1) 담체 선발 및 적정 혼합 비율 탐색 19
2) M. thaumasium WHJ8의 포자 형성도 시험 20
3) M. thaumasium WHJ8의 제제화 20
4. M. thaumasium. WHJ8의 효과 및 품질 기준 확립 21
1) 포트 시험 21
2) 농가 포장 시험 21
3) M. thaumasium WHJ8의 품질 기준 확립 21
Ⅳ. 結果및 考察 23
1. Monacrosporium thaumasium WHJ8의 배양 조사 23
1) 최적 액체배지 선발 및 배양 조건 탐색 23
2) 최적 고체배지 선발 및 배양 조건 탐색 25
2. M. thaumasium WHJ8의 제제화 및 대량 배양 29
1) 담체 선발 및 적정 혼합 비율 탐색 29
2) M. thaumasium WHJ8의 포자 형성도 시험 31
3) M. thaumasium WHJ8의 대량배양 33
3. M. thaumasium WHJ8의 효과 시험 및 품질기준 확립 35
1) 하우스내 포트 시험 35
2) 농가 포장 시험 36
3) M. thaumasium WHJ8의 품질 기준 확립 38
Ⅴ. 綜合考察 40
Ⅵ. 摘要 43
引用文獻 45
Abstract 54
감사의 글 57
Table 1. The effect of media on the conidia production of M. thaumasium WHJ8 after culture 5 day at 25℃. 26
Table 2. The effect of V8 juice concentration on the conidia production of M. thaumasium WHJ8 after culture 5 day at 25℃.(이미지참조) 26
Table 3. Conidia production of M. thaumasium WHJ8 in 10% V8 juice media containing different carbohydrates at 25℃ for 5 days.(이미지참조) 27
Table 4. Conidia production of M. thaumasium in 10% V8 juice media containing different nitrogen compounds at 25℃ for 5 days.(이미지참조) 28
Table 5. Conidia production of M. thaumasium WHJ8 in 10% V8 juice media containing different source of trace element at 25℃ for 5 days.(이미지참조) 28
Table 6. Conidia production of M. thaumasium WHJ8 according to organic and inorganic carrier. Conidia formation were carried out in glass Petri dish, each carrier 50 g containing basal medium at 25℃ for 5 days.(이미지참조) 29
Table 7. Conidia production of M. thaumasium WHJ8 by mixing ratio of organic and inorganic carrier. Conidia formation were carried out in glass Petri dish at 25℃ for 5 days. 30
Table 8. Conidia production of M. thaumasium WHJ8 by different inoculation methods. 32
Table 9. Conidia production and contamination ratio of M. thaumasium WHJ8 under macro-culture conditions. 34
Table 10. Control effect of M. thaumasium WHJ8 formulation to root knot nematode in tomato (cv. Seo-gwang) in pot. survey after 60day in green house. 35
Table 11. Control effect of M. thaumasium WHJ8 formulation to root knot nematode in oriental melon green house. 37
Table 12. Stability test of M. thaumasium WHJ8 formulation under different storage conditions. 38
Table 13. Weight of rat after oral administration of M. thaumasium WHJ8 formulation. 39
Fig. 1. Effect of different media(PDB; potato dextrose broth, CZ; Czapek-Dox broth, PCB; potato carrot broth, MEB; malt extract broth) for M. thaumasium WHJ8 growth in shaking incubator of 140 rpm at 25℃ for 5 days. Cell dry weight was measured 48 hr after in... 23
Fig. 2. Effect of temperature for M. thaumasium WHJ8 growth on PDB medium in shaking incubator of 140 rpm for 7 days. Cell dry weight was measured 48 hr after in dry oven. 24
Fig. 3. Effect of pH for M. thaumasium WHJ8 growth on potato dextrose broth medium in shaking incubator of 140 rpm at 25℃ for 5 days. Cell dry weight was measured 48 hr after in dry oven. 25
Fig. 4. Conidia formation by different inoculation methods. 33
Fig. 5. M. thaumasium WHJ8 formulation for solid-state fermentor. 34
Fig. 6. Effect of M. thaumasium WHJ8 formulation to root knot nematode in tomato (cv. Seo-gwang) in pot. 36
Fig. 7. Oriental melon(cv. Obokkul) after M. thaumasium WHJ8 formulation treatment. 37