This research was performed to investigate the effect of hydrogen peroxide on the stain removal in japanese red pine and japanese hackberry. Sodium silicate and sodium hydroxide were used as activators. Response surface method (RSM) was used to optimize the bleaching conditions such as reaction temperature, reaction time and concentration of hydrogen peroxide and fifteen different bleaching conditions were selected according to 2³ factorial central composite design (CCD). The bleaching effect were evaluated by lightness differences of wood surface before and after the treatment. Lightness differences were measured by a chromameter. In general, lightness differences were increased with the increase of reaction conditions. The R² values of RSM models for japanese pine and japanese hackberry were 0.96 and 0.93, respectively, showing the RSM models represents the bleaching effect. The most affecting factor on the stain removal was concentration of hydrogen peroxide, followed by time and temperature. Second degree of concentration was proved to have an effect on the bleaching at the 0.05% significant levels. The bleaching effect was decreased with the increase of concentrations of hydrogen peroxide. Japanese red pine was difficult to be bleached using hydrogen peroxide compared to japanese hackberry, resulting from the different anatomical properties. This results shows that the optimum bleaching conditions varied according to species. Because the effect of hydrogen peroxide is poor at room temperature, more research is needed to develop the way to improve the bleaching ability at room temperature for the practical purpose.