표제지
목차
Abbreviations 8
Ⅰ. 서론 9
Ⅱ. 재료 및 방법 11
1. 공시재료 및 재배방법 11
2. 실험설계 및 조사방법 13
1) 실험설계 13
2) 생육 및 미질조사 13
3. DNA 추출 및 ISSR-PCR 13
4. RT-PCR 분석 14
Ⅲ. 결과 및 고찰 16
1. 계통별 입본수(立本數)에 따른 양적 형질의 변동 16
2. 기비량(基肥量)에 따른 계통별 양적 형질의 변동 21
3. 계통별 ISSR-PCR과 RT-PCR 분석 26
Ⅳ. 결론 35
참고문헌 36
Abstract 38
감사의 글 40
Table 1. Primers used inter-simple sequence repeat (ISSR) 14
Table 2. ISSR primer sequences (from the University of British Columbia) 14
Table 3. Alanin amino transferase specific primer 15
Table 4. Relative oxygen species specific primer 15
Table 5. Plant height of a cultivar and the different lines of rice 20
Table 6. Main composition of the different lines of unpolished rice 26
Fig. 1. The pedigree diagram of new lines selected from native glutinous rice. 12
Fig. 2. Changes of tillering number in rice lines transplanted with one seedlings as treated with no fertilizer in paddy field. 17
Fig. 3. Changes of tillering number in rice lines transplanted with 2 seedlings as treated with no fertilizer in paddy field. 17
Fig. 4. Changes of tillering number in rice lines transplanted with 3 seedlings as treated with no fertilizer in paddy field. 18
Fig. 5. Changes of tillering number in rice lines transplanted with 7 seedlings as treated with no fertilizer in paddy field. 18
Fig. 6. Tillering number of differnet line, Boseokchalbyeo, GG-05-03, GG-05-04 and GG-05-07, and varied fertilizer. 19
Fig. 7. Changes of plant height of different level of hill in GG-05-03 and compound fertilizer N-P-K:21-17-17% (w/w). 20
Fig. 8. Plant height and grain weight per ear of different line, Boseokchalbyeo, GG-05-03, GG-05-04 and GG-05-07, and varied fertilizer. 23
Fig. 9. Grain number per ear and percent of fertile grain of different line, Boseokchalbyeo, GG-05-03, GG-05-04 and GG-05-07, and varied fertilizer. 24
Fig. 10. 1000-grain weight and grain yield per 10a of different line, Boseokchalbyeo, GG-05-03, GG-05-04 and GG-05-07, and varied fertilizer. 25
Fig. 11. Amplification products obtained from four varieties of Oryza sativa using ISSR primers. 28
Fig. 12. Genomic DNA ISSR banding pattern generated from UBC primers. 28
Fig. 13. Sequence alignment of the most highly conserved region of the unknown homologues. The alignment was done using 'Genetyx'. 29
Fig. 14. Sequence alignment of the most highly conserved region of the AP2 domain homologues. The alignment was done using 'Genetyx'. 29
Fig. 15. RT-PCR analysis of alanine amino transferase family gene expression in Boseokchalbyeo, GG-05-03, GG-05-04 and GG-05-07. 31
Fig. 16. RT-PCR analysis of catalases (CAT A, B, C) expression in Boseokchalbyeo, GG-05-03, GG-05-04 and GG-05-07. 32
Fig. 17. RT-PCR analysis of superoxide dismutase (cSOD, SOD2) expression in Boseokchalbyeo, GG-05-03, GG-05-04 and GG-05-07. 32
Fig. 18. RT-PCR analysis of ascorbate peroxidase (tAPX, sAPX, OsAPX and OsAPX1) expression in Boseokchalbyeo, GG-05-03, GG-05-04 and GG-05-07. 33