표제지
목차
Ⅰ. 서론 8
Ⅱ. 연구사 13
Ⅲ. 재료 및 방법 18
1. 공시 동물 및 조직 채취 18
2. 2-Dimensional Electrophoresis 19
1) 단백질 추출 19
2) 1차원 전기영동 (IEF) 19
3) 2차원 전기영동 (SDS-PAGE) 20
4) 젤 염색 20
3. 단백질 발현 양상 분석 21
4. 단백질 동정 21
1) 질량 분석을 위한 단백질 절편화 21
2) PMF를 이용한 단백질 동정 22
3) CAF(Chemical Association Fragm ent)-MALDI sequencing을 위한 SPITC 반응 22
4) CAF-MALDI sequencing을 이용한 단백질 동정 23
Ⅳ. 결과 및 고찰 24
실험 1. Landrace와 Berkshire간 등심조직의 단백체 분석 24
실험 2. 성장 단계 별 등심 조직의 단백체 연구 33
실험 3. 인진쑥 분말 첨가 급여 돼지의 단백체 연구 41
Ⅴ. 요약 50
Ⅵ. 참고 문헌 53
Abstract 66
Table 1. Meat quality traits of the loin in different pig breeds 9
Table 2. Analysis of the loin protein, differentially expressed between Berkshire and Landrace. 30
Table 3. Loin proteins exhibiting differentially expression during development. 39
Table 4. Identification by PMF and CAF-MALDI sequencing of proteins exhibiting a change of expression following a dietary treatment of mugwort diet. 47
Figure 1. Flow chart of the proteomics. 10
Figure 2. Two-dimentional (2-D) gel images of the Longissimus dorsi muscle. 25
Figure 3. Comparison of 2-D gel images for loin proteins between Landrace (upper panel) and Berkshire (lower panel). 26
Figure 4. Analysis of the individual proteins which have exhibited differentional expression between Landrace and Berkshire. 28
Figure 5. Comparison of protein expression amounts in pork loin by different growth stages. 34
Figure 6. Comparison of 2D-gel images loin proteins between Berkshire (upper panels) at the finishing stage and Berkshire (lower panels) during the growth stage. 35
Figure 7. Analysis of the individual proteins which have exhibited differential expression between growth stage and finishing stage. 37
Figure 8. Two-dimentional gel images for loin proteins of the pig fed a basal diet (12 panel) on the basal diet supplementated with 1.5% mugwort powder. 42
Figure 9. Comparison of 2-D gel images for loin proteins between basal diet (upper panel) and the basal diet supplemented with 1.5% mugwort powder (lower panel). 43
Figure 10. Analysis of the individual proteins which have exhibited differentional expression between basal diet and the basal diet supplemented with 1.5% mugwort powder. 45