표제지
목차
Ⅰ. 서론 10
Ⅱ. 연구사 12
1. 염색체 12
2. 혈액세포로 부터 염색체 분리 방법 12
1) 혈액세포의 구성 및 특징 12
2) 혈액세포를 이용한 염색체 분리 13
3. 동물 염색체 분석 16
1) 포유류 16
2) 조류 17
Ⅲ. 재료 및 방법 19
1. 공시동물 19
2. 혈액세포배양을 이용한 염색체 분리 19
1) 혈액세포 배양 19
2) 염색체 유도 21
3) 저장처리 21
4) 고정처리 22
5) 표본제작 22
6) 염색체 분석 방법 22
7) 통계분석 23
Ⅳ. 결과 및 고찰 24
1. 소의 염색체 분리 24
2. 닭의 염색체 분리 31
3. 랫드의 염색체 분리 37
4. 마우스의 염색체 분리 43
Ⅴ. 결론 49
Ⅵ. 참고문헌 51
Abstract 59
Table 1. Analysis of variances for the factors affecting to the mitotic index of bovine lymphocytes 28
Table 2. Analysis of variances for the factors affecting to the chromosome morphology of bovine lymphocytes 29
Table 3. Mitotic index and chromosome morphology of bovine lymphocytes according to culture duration, mitogen supplement and colcemid exposure 30
Table 4. Analysis of variances for the factors affecting to the mitotic index of chicken lymphocytes 34
Table 5. Analysis of variances for the factors affecting to the chromosome morphology of chicken lymphocytes 35
Table 6. Mitotic index and chromosome morphology of chicken lymphocytes according to culture duration, mitogen supplement and colcemid exposure 36
Table 7. Analysis of variances for the factors affecting to the mitotic index of rat lymphocytes 40
Table 8. Analysis of variances for the factors affecting to the chromosome morphology of rat lymphocytes 41
Table 9. Mitotic index and chromosome morphology of rat lymphocytes according to culture duration, mitogen supplement and colcemid exposure 42
Table 10. Analysis of variances for the factors affecting to the mitotic index of mouse lymphocytes 46
Table 11. Analysis of variances for the factors affecting to the chromosome morphology of mouse lymphocytes 47
Table 12. Mitotic index and chromosome morphology of mouse lymphocytes according to culture duration, mitogen supplement and colcemid exposure 48
Figure. 1. Metaphase spreads of bovine lymphocytes; Chromosome morphology was divided by five grades according to the patterns of chromosome shape ; (A) Very poor shape(scoring 1), (B) Poor shape(scoring 2), (C) Common shape(scoring 3), (D) Good shape(scoring 4) and (E) Excellent shape(scoring 5) 27
Figure 2. The mitotic index of bovine lymphocytes in different culture condition 28
Figure 3. The score of chromosome morphology of bovine lymphocytes in different culture condition 29
Figure. 4. Metaphase spreads of chicken lymphocytes; Chromosome morphology was divided by five grades according to the patterns of chromosome shape ; (A) Very poor shape(scoring 1), (B) Poor shape(scoring 2), (C) Common shape(scoring 3), (D) Good shape(scoring 4) and (E) Excellent shape(scoring 5) 33
Figure 5. The mitotic index of chicken lymphocytes in different culture condition 34
Figure 6. The score of chromosome morphology of chicken lymphocytes in different culture condition 35
Figure. 7. Metaphase spreads of rat lymphocytes; Chromosome morphology was divided by five grades according to the patterns of chromosome shape ; (A) Very poor shape(scoring 1), (B) Poor shape(scoring 2), (C) Common shape(scoring 3), (D) Good shape(scoring 4) and (E) Excellent shape(scoring 5) 39
Figure 8. The mitotic index of rat lymphocytes in different culture condition 40
Figure 9. The score of chromosome morphology of rat lymphocytes in different culture condition 41
Figure. 10. Metaphase spreads of mouse lymphocytes; Chromosome morphology was divided by five grades according to the patterns of chromosome shape ; (A) Very poor shape(scoring 1), (B) Poor shape(scoring 2), (C) Common shape(scoring 3), (D) Good shape(scoring 4) and (E) Excellent shape(scoring 5) 45
Figure 11. The mitotic index of mouse lymphocytes in different culture condition 46
Figure 12. The score of chromosome morphology of mouse lymphocytes in different culture condition 47