Title Page
Abstract
Contents
Introduction 8
Methods 12
Neuron culture 12
Drug treatment 12
LDH assay 12
MTS assay 13
LC3 gene plasmid transfection 13
Western blot analysis 13
Lysotracker staining 14
Immunocytochemistry 14
Transmission electron microscopy(EM) 14
Statistical Analysis 15
Results 16
The effects of antipsychotics on neuron viability 16
Induction of autophagy by antipsychotics 16
Autophagy via immunostaining of LC3 20
Verification of mechanism of LC3-II accumulation using bafilomycin A1 20
Autophagosome accumulation investigated with Electron microscopy 23
Colocalization of autophagosome and lysosome using fluorescence microscopy 23
Discussion 30
Conclusion 33
References 34
한글 초록 40
Fig 1. Negative effects of antipsychotics on neuron viability (LDH assay) 17
Fig 2. Negative effects of antipsychotics on neuron viability (MTS assay) 18
Fig 3. Concentration- and time-dependent accumulation of LC3-II induced by antipsychotics 19
Fig 4. Visual confirmation of induction of autophagy via immunostaining of LC3 21
Fig 5. Verification of mechanism of LC3-II accumulation using bafilomycin A1 (western blot) 22
Fig 6. Autophagic accumulation in cortical neurons treated with Haloperidol, Clozapine via EM. 25
Fig 7. Colocalization of autophagosome and lysosome using fluorescence microscopy 27
Fig 8. Tandem monomeric GFP-mCherry-LC3 transfection with clozapine, haloperidol 28