표제지
목차
요약 7
서론 9
실험 재료 및 방법 16
Cell culture 및 virus infection 16
Plasmids 16
Transfection 17
Antibodies 17
Co-immunoprecipitation assays 18
Immunoblot analysis 18
Indirect immunofluorescence assay (IFA) 19
Luciferase reporter assay 19
실험 결과 21
1. ZBP1과 RIP1의 상호작용 21
2. ZBP1과 RIP1의 세포 내 분포 21
3. ZBP1과 RIP1의 colocalization 22
4. 293T 세포에서 NF-κB 활성화에 대한 ZBP1-RIP1 상호작용의 역할 33
5. HepG2 세포에서 NF-κB 활성화에 대한 ZBP1-RIP1 상호작용의 역할 34
고찰 44
참고 문헌 48
ABSTRACT 53
Fig. 1. Illustration of DNA-mediated activation of ZBP1 and downstream signaling molecules. 11
Fig. 2. Schematic representation of ZBP1 and RIP1. 14
Fig. 3. Interaction of ZBP1 with RIP1 through their RHIMs 23
Fig. 4. Cytoplasmic distribution of RIP1 (wt or △RHIM) and ZBP1 in 293T cells 25
Fig. 5. Localization patterns of RIP1 (wt or △RHIM) and ZBP1 in HepG2 cells. 27
Fig. 6. Colocalization of ZBP1 and RIP1 in 293T cells. 29
Fig. 7. Colocalization interaction of ZBP1 with RIP1 in Hep G2 cells. 31
Fig. 8. Role of the ZBP1-RIP1 interaction on NF-κB activation in 293T cells 36
Fig. 9. Role of the ZBP1-RIP1 interaction on NF-κB activation in B-DNA-stimulate d293T cells 38
Fig. 10. Role of the ZBP1-RIP 1 interaction on NF-κB activation in Hep G2 cells 40
Fig. 11. Role of the ZBP1-RIP 1 interaction on NF-κB activation in HepG2 cells stimulated with B-DNA or infected with HSV-1 42