표제지
목차
국문초록 14
Abstract 17
I. 서론 20
1. 연구배경 20
1) 목초액 이용 연혁(沿革) 20
2) 목초액의 품질 22
3) 목초액의 성분 24
4) 목초액의 안전성 지침 28
5) 산업적 용도로 본 목초액 29
6) 자연치유적 이용 및 연구동향 32
2. 표재성 진균증(superficial mycoses) 41
1) 피부와 미생물(皮膚와 微生物) 41
2) 진균의 형태와 분류 43
3) 표재성 피부진균증(皮膚眞菌症) 특성 44
4) 발병기전 및 원인 44
5) 항진균요법(Antifungal therapy)과 목초액의 표재성진균증 치유 47
II. 연구의 필요성 및 목적 52
1. 연구의 필요성 52
2. 연구의 목적 55
III. 재료 및 방법 56
1. 항진균 및 항세균 활성검정 56
1) 균주 및 배양 56
2) 실험용 목초액 57
3) 디스크 확산법 57
4) 실험균 접종액 준비 58
5) 실험균의 액체배지 대량 희석법에 따른 최소억제 농도(MIC) 측정 59
2. 목초액에 의한 표재성 진균증 임상 61
1) 연구대상 61
2) 목초액의 제조방법 62
3) 목초액의 선별 68
4) 임상방법 68
3. 설문조사방법 71
1) 작성모델 71
2) 구성 71
3) 점수와 채점 71
4. 자료 통계적 분석 71
IV. 연구결과 74
1. 항진균 및 항진균 활성검정결과 74
1) 디스크 확산법에 의한 증식억제 효과 74
2) 액체배지에서 최소억제 농도(MIC) 측정 78
2. 목초액 임상결과 94
1) 연구 대상자의 일반적 특성 94
2) 연구대상자 연구 집단별 분류 95
3) 목초액의 표재성진균증에 대한 임상효과 96
V. 고찰 134
VI. 결론 154
참고문헌 158
부록 174
〈부록 1〉 목초액의 무좀치료 임상실험에 대한 협조요청 174
〈부록 2〉 목초액의 표재성진균증 치료 임상일지(양식) 176
〈부록 3〉 설문서(양식) 178
〈부록 4〉 Table 1. 제조법에 따른 목초액의 품질기준 180
〈부록 5〉 Table 2. Vinegar for antifungal and antibacterial activities 180
Table 1. List of microorganisms used in this study 56
Table 2. Wood vinegar samples used as antifungal and antibacterial agent... 57
Table 3. Water acquity MPLC system 60
Table 4. Micromass Q-TOF Premier mass spectrometer 61
Table 5. Classification of clinical subjects 62
Table 6. Relation among smoke color, temperature and carburetion... 65
Table 7. Classification of experimental subject, clinic method and times 69
Table 8. The inhibition zone of bacterial growth to the wood vinegar 76
Table 9. MICs of wood vinegar sample dilutions to S. aureus 1621 82
Table 10. MICs of wood vinegar dilutions to E. coli 2517 82
Table 11. MICs of wood vinegar dilutions to A. baumannii 2508 82
Table 12. MICs of wood vinegar dilutions to B. epidermidis 3098 83
Table 13. MICs of wood vinegar dilutions to C. xerosis 3435 83
Table 14. MBCs of wood vinegar samples to S. aureus 1621 84
Table 15. MBCs of wood vinegar samples to E. coli 2571 85
Table 16. MBCs of wood vinegar samples to A. baumannii 2508 85
Table 17. MBCs of wood vinegar samples to B. epidermidis 3098 85
Table 18. MBCs of wood vinegar samples to C. xerosis 3435 86
Table 19. MICs of wood vinegar samples 5 & 2 to B. epidermidis 88
Table 20. MICs of wood vinegar samples 5 & 2 to E. coli 2571 88
Table 21. MICs of wood vinegar samples 5 & 2 to A. baumannii 89
Table 22. MICs of wood vinegar samples 5 & 2 to S. aureus 1621 89
Table 23. MICs of wood vinegar samples 5 & 2 to C. xerosis 3435 90
Table 24. MICs of wood vinegar samples 2 fractions to B. epidermidis 3089 91
Table 25. MBCs of wood vinegar samples-2 fractions to B. epidermidis 3089 91
Table 26. Classification and symptoms of experimental subjects 94
Table 27. Characteristics of experimental groups 96
Table 28. Overall data of healing by the wood vinegar treatments... 98
Table 29. Overall data of healing by the wood vinegar treatments... 102
Table 30. Overall data of healing by the wood vinegar treatments... 106
Table 31. Summary of healing effects of A, B and C groups 108
Table 32. Healing effects of mycoses according to experimental groups 110
Table 33. Healing effects of mycoses according to sex distinction 111
Table 34. Comparison of cure ratios according to ages 112
Table 35. Comparison of cure effects according treating frequency 113
Table 36. Healing effects from before and after treatment in the groups 115
Table 37. Relapse ratios in the A, B and C groups 116
Table 38. Relapse ratios according to sexes after treating 117
Table 39. Differences of symptom disappearance according to groups 119
Table 40. Motivation using wood vinegar for cure of superficial mycoses 120
Table 41. Effects after feet-soaking in the wood vinegar for over 40 minutes... 121
Table 42. Healing effects of mycose symptoms after soaking in the... 123
Table 43. Practical application from feet soaking in wood vinegar... 124
Table 44. Agreeableness degree when feet-soaking in wood vinegar 125
Table 45. Influence to fumigation scent during wood vinegar soaking 126
Table 46. Difference of relapse time after soaking more than... 128
Table 47. Propriety as a treatment material of wood vinegar... 129
Table 48. Recognition degree as a treatment material of wood vinegar... 130
Table 49. Recommendation of wood vinegar as a treatment... 131
Figure 1. Structure of ordinal charcoal kiln (Yangpyung Charcoal). 64
Figure 2. Diagram of vertical sequence carburetion stove... 64
Figure 3. Soaking of feet in the wood vinegar solution. 69
Figure 4. Soaking of feet in the wood vinegar solution in plastic bag. 69
Figure 5. Inhibition tests of samples to... 75
Figure 6. Disk diffusion of wood vinegar samples to bacteria. 77
Figure 7. Growth inhibition of T. rubrum 6345 to wood vinegar sample 2. 78
Figure 8. Growth inhibition of T. rubrum 6345 to the sample 2-2. 79
Figure 9. Growth inhibition of T. rubrum 6345 to the sample 3. 79
Figure 10. Growth inhibition of T. rubrum 6345 to sample 4. 80
Figure 11. Growth inhibition of T. rubrum 6345 to sample 5. 80
Figure 12. Growth inhibition of T. rubrum 6345 to sample 5-1. 81
Figure 13. Chromatography of fractions obtained by silica gel column... 92
Figure 14. Chromatography of fractions obtained by Sephadex column... 92
Figure 15. HPLC of wood vinegar sample 2-chloroform... 93
Figure 16. Analysis usisng the Q-TOF mass spectrophotometer of wood vinegar... 93
Figure 17. Healing effect of superficial mycoses by wood vinegar... 108
Figure 18. Histogram of healing effects of mycoses according to... 110
Figure 19. Healing effects of mycoses according to sex distinction. 111
Figure 20. Histogram of cure ratios according to ages... 112
Figure 21. Healing levels according to treated times in he groups. 114
Figure 22. Relapse ratios before and after treatments in the groups. 117
Figure 23. Disappearances of Erythema, Desquamation and... 119
Figure 24. Motivations using wood vinegar for cure of superficial... 121
Figure 25. Histogram of effects after feet-soaking in the wood vinegar... 122
Figure 26. Histogram of healing effects of mycose symptoms after soaking... 123
Figure 27. Histogram of differences of application from wood vinegar... 125
Figure 28. Agreeableness degree when feet-soaking in wood vinegar. 126
Figure 29. Influence to fumigation scent during wood vinegar soaking. 127
Figure 30. Histogram of difference of relapse time after soaking... 128
Figure 31. Propriety as a treatment material of wood vinegar... 129
Figure 32. Recognition degree as a treatment material of wood vinegar... 130
Figure 33. Recommendation of wood vinegars as a treatment... 131