Title Page
Contents
ABSTACT 6
I. INTRODUCTION 8
II. MATERIALS AND METHODS 10
1. Cell preparation 10
2. Animal model 11
3. Transplantation of cells 12
4. Tissue preparation 12
5. Morphometry 12
6. Cell death 13
7. Immunohistochemistry 13
8. Enzyme-linked immunosorbent assay (ELISA) 14
9. In vitro cell culture 14
10. Statistical analysis 15
III. RESULTS 18
1. Alveolarization 18
2. Angiognesis 18
3. Cell death and pulmonary inflammation 19
4. In vitro expression of growth factors 20
IV. DISCUSSION 29
V. CONCLUSION 33
REFERENCES 34
ABSTRACT (Korean) 40
Fig. 1. Comparison of mesenchymal stem cells from umbilical cord blood and fat tissue 16
Fig. 2. Experimental schedule 17
Fig. 3. Histology and morphometric analysis in the lungs 22
Fig. 4. Pulmonary angiogenesis 23
Fig. 5. Cell death and active macrophages in the lungs 24
Fig. 6. Inflammatory cytokine proteins in the lungs 26
Fig. 7. In vitro expression of growth factors and cell vaibility 27