표제지
목차
I. 서론 10
II. 재료 및 방법 20
1. 실험재료 20
1) 시료 20
2) 사용 균주 26
3) 균주의 배양 26
2. 실험 방법 29
1) 아이 섀도우의 오염도 측정(무 방부제 & 파라벤) 29
2) 3M™ petrifilm 측정 31
3) 항균활성 측정(MIC) 32
4) 아이 섀도우 오염도 측정(봉독) 34
5) 챌린지 테스트(Challenge test) 34
III. 결과 및 고찰 36
1. 무 방부제와 파라벤 첨가 아이 섀도우 오염도 36
2. 파라벤 함유한 아이 섀도우에 검출된 미생물 확인 42
3. 봉독 MIC(최소억제농도) 확인 44
4. 봉독 첨가에 따른 아이 섀도우 미생물 억제 효과 44
5. 봉독을 첨가한 아이 섀도우 챌린지 테스트 47
IV. 요약 64
V. 참고문헌 67
Abstract 73
Table 1. Bee venom ingredient 21
Table 2. Bee venom property 22
Table 3. Formulation of eye shadow samples 24
Table 4. The classification of strain used in experimental... 28
Table 5. Composition of bacteria and fungi culture medium 30
Table 6. 3M™ petrifilm culture conditions and strains decipher 33
Table 7. 3M™ Petrifilm microorganisms check results 43
Table 8. Minimum inhibitory concentration(MIC) of... 45
Fig. 1. Microbial contamination of eye shadow 12
Fig. 2. The chemical structure of germicidal synthetic preservatives 14
Fig. 3. Chemical structure of bee venom 17
Fig. 4. Photograph of bee venom 23
Fig. 5. Photograph of eye shadow 27
Fig. 6. Confirmation of bacteria contamination in preservative... 37
Fig. 7. Confirmation of fungi contamination in preservative... 39
Fig. 8. Confirmation of bacteria contamination in paraben... 40
Fig. 9. Confirmation of fungi contamination in paraben... 41
Fig. 10. Minimum inhibitory concentration(MIC) of the... 46
Fig. 11. Confirmation of bacteria contamination in eye... 48
Fig. 12. Confirmation of fungi contamination in eye... 49
Fig. 13. Confirmation of bacteria contamination in eye... 50
Fig. 14. Confirmation of fungi contamination in eye... 51
Fig. 15. Confirmation of bacteria contamination in eye... 52
Fig. 16. Confirmation of fungi contamination in eye... 53
Fig. 17. Confirmation of bacteria contamination in eye... 54
Fig. 18. Confirmation of fungi contamination in eye... 55
Fig. 19. Confirmation of bacteria contamination in eye... 56
Fig. 20. Confirmation of fungi contamination in eye... 57
Fig. 21. Growth of E. coli in eye shadow containing 0.1%,... 59
Fig. 22. Growth of S. aureus in eye shadow containing... 60
Fig. 23. Growth of B. subtilis in eye shadow containing... 62
Fig. 24. Growth of C. albicans in eye shadow containing... 63