표제지
목차
제1장 서론 7
제2장 재료 및 방법 9
1. 연구 재료 9
2. 배양 방법 12
3. 분리된 세균의 동정 13
4. 항생제 감수성 시험 14
5. 분리된 Methicillin resistant S. aureus로부터 genomic DNA 분리 16
6. mecA, mecB 유전자 검출 및 SCCmec과 mec complex typing 17
7. DNA 전기영동 19
제3장 결과 21
1. 軍 훈련병에서의 세균 분리 21
2. Methicillin-resistant S. aureus (MRSA)의 분리 동정 23
3. Methicillin-resistant S. aureus (MRSA)의 항생제 감수성 시험 25
4. Methicillin-resistant S. aureus (MRSA)의 mecA 유전자 검출 및 SCCmec 과 mec complex type 분석 28
제4장 고찰 36
참고문헌 40
Abstract 43
Table 1. Number of specimens collected from healthy army trainees and... 10
Table 2. Specific oligonucleotide primer sets used for detection of mecA gene, and subtyping of SCC mec... 20
Table 3. Bacterial isolates from nasal swab of healthy army trainees 22
Table 4. Isolation of Staphylococcus spp. from healthy army trainees 24
Table 5. Antibiotic susceptibility patterns of MRSA isolated from healthy army trainees and clinical patients 26
Table 6. Subtyping of Staphylococcal cassette chromosome mec (SCCmec) and mec complex from healthy... 30
Table 7. Comparison of antibiotic resistance phenotypic profiles and genotypes of MRSA isolates 32
Figure 1. Example of Hand plate to collect specimens from... 11
Figure 2. MRSA detection and procedures for inspection 15
Figure 3. Multiplex PCR assay identifies SCCmec types and subtypes... 34
Figure 4. Multiplex PCR assay identifies SCCmec types and subtypes... 34
Figure 5. Multiplex PCR assay identifies SCCmec types and subtypes... 35