Title Page
ABSTRACT
Contents
List of Abbreviations 12
INTRODUCTION 15
MATERIALS AND METHODS 20
Preparation of MSA and kits for 99mTc labeling(이미지참조) 21
In vitro visibility test of dyes 22
Absorption spectra and molar absorption coefficient (ε) of MSA-dye conjugates 23
In vitro measurements for the binding efficiencies of dyes with MSA 23
Size exclusion HPLC before and after MSA and dye conjugation 26
Electrophoresis for the binding mechanism study of MSA and dyes 26
In vitro fluorescence monitoring of MSA-dye conjugates 27
In vivo visible and fluorescence experiments of MSA-NBB conjugate for the detection of SLN 30
Preparation for 99mTc-MSA-NBB conjugate(이미지참조) 30
Analysis of SPECT/CT 31
Stability test of 99mTc-MSA-NBB conjugate in in vivo(이미지참조) 32
RESULTS 34
In vitro visibility test of dyes 34
Absorption spectra and molar absorption coefficient (ε) of MSA-dye conjugates 36
In vitro measurements for the binding efficiencies of dyes with MSA 40
Size exclusion HPLC before and after MSA and dye conjugation 45
Electrophoresis for the binding mechanism study of MSA and dyes 49
In vitro fluorescence monitoring of MSA-dye conjugates 51
In vivo visible and fluorescence experiments of MSA-NBB conjugate for the detection of SLN 53
Preparation for 99mTc-MSA-NBB conjugate(이미지참조) 57
Analysis of SPECT/CT 62
Stability test of 99mTc-MSA-NBB conjugate in in vivo(이미지참조) 64
DISCUSSION 66
CONCLUSION 73
REFERENCES 74
국문초록 83
TABLE 1. Various conditions of mobile phase for TLC. 25
TABLE 2. Wavelength ranges for in vitro fluorescent imaging. 29
TABLE 3. Calculation of molar absorption coefficient (ε) using OD value of MSA-dye... 38
TABLE 4. Binding efficiencies of MSA and dyes. 41
TABLE 5. Dye/MSA ratio (mol/mol) calculated by TLC experiments (MSA: 71021). 44
FIGURE 1. Molecular structure of dyes. 19
FIGURE 2. In vitro visibility tests of dyes. 35
FIGURE 3. Absorption spectra of MSA-dye conjugates. 37
FIGURE 4. Wavelength differences of dye and MSA-dye conjugate in UV-VIS-NIR spectra. 39
FIGURE 5. Binding efficiencies of MSA and dyes. 43
FIGURE 6. Size exclusion HPLC chromatograms of protein standards (A) and MSA-dye... 48
FIGURE 7. Electrophoresis results of MSA-dye conjugates for the binding mechanism study. 50
FIGURE 8. Fluorescence monitoring of MSA (0.625-20 mg) and dye conjugates. 52
FIGURE 9. In vivo visible and fluorescence experiments of MSA-NBB conjugate for SLN... 56
FIGURE 10. Radiochemical purity of 99mTc-MSA determined by radio-TLC (ITLC).(이미지참조) 58
FIGURE 11. Particle size of HSA, MSA, MSA kit (reduced MSA), and 99mTc-MSA analyzed by...(이미지참조) 59
FIGURE 12. Size exclusion HPLC chromatograms of protein standards (A) 99mTc-MSA (B).(이미지참조) 61
FIGURE 13. SPECT/CT after 99mTc-MSA-NBB conjugate injection into the left footpad of a...(이미지참조) 63
FIGURE 14. In vivo stability tests of 99mTc-MSA-NBB conjugate.(이미지참조) 65