Title Page
Contents
I. INTRODUCTION 10
1. Polyphasic Taxonomy 10
1.1. Genotypic Taxonomy 10
1.2. Chemotaxonomy 11
1.3. Phenotypic Taxonomy 12
2. Report of the genus of bacteria in this research 13
2.1. The genus Flaviaesturariibacter 13
3. Report of the genus of yeast in this research 14
3.1. The genus Mrakia 14
II. METHODS 16
1. Isolation and deposit of bacteria 16
2. Genotypic Taxonomy of bacteria 18
2.1. 16S rRNA gene sequencing analysis 18
2.2. Phylogenetic analysis 18
2.3. Genome sequence analysis 19
3. Chemotaxonomy of bacteria 22
3.1. Fatty acid 22
3.2. Polar lipid and Isoprenoid quinone analysis 24
4. Phenotypic Taxonomy of bacteria 25
4.1. Gram-staining test 25
4.2. Transmission electron & Light Microscopy 25
4.3. Catalase & Oxidase activities test 25
4.4. Growth test and API test 25
5. Isolation and deposit of yeast 27
6. Genotypic Taxonomy of yeast 29
6.1. D1/D2 domain of LSU rRNA gene and ITS region sequencing and phylogenetic analysis 29
7. Phenotypic Taxonomy of yeast 30
7.1. Morphological, physiological and biochemical characteristics 30
III. RESULTS 31
1. Characterization novel species of the genus Flaviaesturariibacter in bacteria 31
1.1. Flaviaesturariibacter aridisoli sp. nov. (strain 17J68-15T)[이미지참조] 31
1.2. Taxonomic conclusion 41
2. Characterization novel species of the genus Mrakia in yeast 42
2.1. Mrakia terrae sp. nov. (strain YP416T)[이미지참조] 42
2.2. Mrakia stelviica sp. nov. (strain YP574) 48
2.3. Taxonomic conclusion 52
IV. DESCRIPTION OF NOVEL SPECIES 53
1. Description of Flaviaesturariibacter aridisoli sp. nov. 53
2. Description of Mrakia terrae sp. nov. 55
3. Description of Mrakia stelviica sp. nov. 56
V. REFERENCES 57
ABSTRACT 64
국문요약 66
Table 1. Information of isolated strain and reference strains 17
Table 2. Information of isolated yeast strains 28
Table 3. Cellular fatty acid profiles of Flaviaesturariibacter aridisoli... 35
Table 4. Differential characteristics of Flaviaesturariibacter aridisoli... 39
Table 5. Nucleotide substitutions and sequence similarity (%, in... 43
Table 6. Nutrient assimilation that differentiate Mrakia terrae YP416T...[이미지참조] 47
Fig. 1. iSeq 100 sequencer (Illumina, USA) 20
Fig. 2. Nanopore MinION device (Oxford Nanopore Technologies, England) 21
Fig. 3. Sample preparation for fatty acid profile analysis 23
Fig. 4. Neighbor-joining tree based on 16S rRNA gene sequence of... 34
Fig. 5. Polar lipid profile of strain 17J68-15T, after two-dimensional...[이미지참조] 36
Fig. 6. Isoprenoid quinone analysis of strain 17J68-15T using high...[이미지참조] 37
Fig. 7. Cell morphology of strain 17J68-15T observed by energy-filtering...[이미지참조] 38
Fig. 8. Phylogenetic tree based on the LSU (D1/D2 domain) rRNA gene... 44
Fig. 9. Maximum likelihood tree based on the LSU (D1/D2 domain) rRNA... 45
Fig. 10. Morphologies of Mrakia terrae YP416T observed by phase...[이미지참조] 46
Fig. 11. Phylogenetic tree based on the LSU (D1/D2 domain) rRNA gene... 49
Fig. 12. Cell morphologies of Mrakia stelviica YP574 observed by phase... 50
Fig. 13. True hyphae of Mrakia stelviica YP574 observed by phase... 51