Title Page
Contents
I. INTRODUCTION 8
1. Report of the genus Paenibacillus of bacteria 8
2. Report of the genus Mrakia of yeast 9
3. Polyphasic Taxonomy 10
4. Illumina iSeq 100 12
II. MATERIAL & METHODS 14
1. Bacteria Paenibacillus alboflavus sp. nov. 14
1.1. Isolation of bacterial strain from soil 14
1.2. Genome sequencing and genotypic taxonomy 14
1.3. Molecular Taxonomy 19
1.4. Chemotaxonomy 20
1.5. Morphology and physiological classification 24
2. Yeast Mrakia soli sp. nov. 26
2.1. Isolation of yeast from soil 26
2.2. Molecular taxonomy and phylogenetic analysis 26
2.3. Morphology and physiological classification 29
III. RESULT 30
1. Paenibacillus alboflavus sp. nov. 30
1.1. Phylogenetic and genome analyses 30
1.2. Morphological and phygiological characteristics 37
1.3. Chemotaxonomy 42
2. Mrakia soli sp. nov. 46
2.1. Molecular phylogenetics 46
2.2. Morphology and physiological classification 46
IV. DISCUSSION 52
1. Taxonomic conclusion of Paenibacillus alboflavus sp. nov. 52
2. Taxonomic conclusion of Mrakia soli sp. nov. 52
V. REFERENCES 54
ABSTRACT 61
국문요약 63
Table 1. List of reference strains with high similarity to starin 18JY21-1T.[이미지참조] 32
Table 2. The genomic characterization of strain 18JY21-1T along with related species, along with GGDC and ANI values were...[이미지참조] 34
Table 3. Differential characteristics between strain 18JY21-1T and closely related...[이미지참조] 41
Table 4. Cellular fatty acid profiles of strain 18JY21-1T and closely related species....[이미지참조] 43
Table 5. Number of nucleotide substitutions and sequence similarity (%, in... 48
Table 6. Nutrient assimilation that differentiate Mrakia soli from the closest related... 50
Fig 1. D1/D2 domains of Large Subunit (LSU) rRNA gene position and primer. 27
Fig 2. Internal transcribed spacer (ITS) region and primer. 28
Fig 3. Phylogenetic tree based on the 16S rRNA gene sequences of strain... 33
Fig 4. Heatmap generated with OrthoANI values calculated from the OAT software. 35
Fig 5. The genome map of strain 18JY21-1T obtained from the ezbiocloud web site.[이미지참조] 36
Fig 6. Morphology of cells of strain 18JY21-1T in Transmission electron...[이미지참조] 39
Fig 7. Microscopic images of strain 18JY21-1T. The endospores of on Schaeffer's...[이미지참조] 40
Fig 8. Isoprenoid quinones analysis of strain 18JY21-1T. (A), MK-6,7 standard; (B),...[이미지참조] 44
Fig 9. Polar lipids analysis of strain 18JY21-1T. (A), total lipids; (B), phospholipids;... [이미지참조] 45
Fig 10. Phylogenetic tree based on the D1/D2 domains LSU rRNA gene and ITS...[이미지참조] 49
Fig 11. Morphologies of Mrakia soli YP421T. Cells were grown on YM agar for 3...[이미지참조] 51