표제지
목차
I. Inactivation of Salmonella and Listeria monocytogenes in steamed fish paste using an integrated treatment of malic acid-incorporated edible coating and in-package cold plasma treatment 16
1. 서론 17
2. 재료 및 방법 20
2.1. 어묵 시료 준비 20
2.2. 미생물 접종원 준비 및 접종 20
2.3. Malic acid를 혼입한 whey protein isolate 코팅 용액 및 필름 제조 21
2.4. Disk covering test 21
2.5. In-package CP 처리 22
2.6. 미생물 분석 25
2.7. 색도 측정 25
2.8. 저장 실험 25
2.9. 어묵에 코팅된 malic acid 혼입-whey protein isolate 필름에서의 malic acid 확산 속도 결정 26
2.10. Malic acid 정량 27
2.11. 통계 분석 27
3. 결과 및 고찰 29
3.1. MA-WPI 필름 형성 및 필름을 이용한 disk-covering test 29
3.2. CP 처리 조건 결정 32
3.3. 색도 32
3.4. MA-WPI 코팅과 CP 처리와의 병합 처리의 Salmonella 와 L. monocytogenes 저해 35
3.5. 병합 처리의 저장 중 Sahuonellad와 L. monocytogenes 생장에 대한 영향 38
3.6. CP 처리의 MA-WPI 필름에서의 malic acid 확산에 대한 영향 42
4. 결론 45
II. Effect of in-package atmospheric dielectric barrier discharge cold plasma treatment on the antimicrobial efficacy of malic acid-incorporating edible films against food-borne pathogens in chicken breast processed meat 46
1. 서론 47
2. 재료 및 방법 50
2.1. 닭가슴살 가공육 시료 준비 50
2.2. 미생물 접종원 준비 및 접종 50
2.3. 코팅 용액 제조 및 필름 제작 50
2.4. In-package CP 처리 51
2.5. 미생물 분석 53
2.6. 저장 실험 53
2.7. Malic acid 확산 54
2.8. 필름 특성 측정 56
2.9. 통계 분석 57
3. 결과 및 고찰 58
3.1. CP 처리 조건 결정 58
3.2. MA-WPI 코팅과 CP 처리의 병합 처리를 이용한 Salmonella와 L. monocytogenes 저해 62
3.3. 저장 중 Salmonella 생장 64
3.4. MA-WPI 필름에서의 malic acid 확산 71
3.5. MA-WPI 필름 특성 81
3.6. 저장 중 이화학적 특성의 변화 84
4. 결론 88
참고 문헌 89
ABSTRACT 102
Table 1. Effects of whey protein isolate coating incorporating malic acid... 34
Table 2. Diffusion coefficients (D₁) of malic acid in whey protein isolate... 44
Table 3. Compositions of whey protein isolate (WPI)-based coating... 52
Table 4. Effect of the glycerol concentration on the diffusion coefficient... 73
Table 5. Effect of the in-package atmospheric dielectric barrier... 76
Table 6. Effect of cold plasma (CP) treatment on water vapor permeability (WVP), elongation at break, elastic... 83
Table 7. The effects of the whey protein isolate coating with malic acid (MA-WPI), in-package atmospheric... 85
Table 8. The effects of the whey protein isolate coating with malic acid... 87
Figure 1. Schematic of the cold plasma treatment system used in this study. 24
Figure 2. Appearance of whey protein isolate film with malic acid. 30
Figure 3. Inhibition zone formation of whey protein isolate (WPI) film (A) and WPI film incorporating 3% (w/w... 31
Figure 4. Effects of whey protein isolate coating with malic acid... 37
Figure 5. Effects of whey protein isolate coating incorporating malic acid... 39
Figure 6. Effects of whey protein isolate coating incorporating malic acid... 41
Figure 7. The release of malic acid from the whey protein isolate based... 43
Figure 8. Effect of the treatment voltage on the inactivation of... 60
Figure 9. Effect of the treatment time on the inactivation of Salmonella... 61
Figure 10. Effects of whey protein isolate coating with malic acid... 63
Figure 11. Effects of the glycerol concentration of the whey protein... 65
Figure 12. Effects of whey protein isolate coating incorporating malic... 67
Figure 13. Effects of whey protein isolate coating incorporating malic... 70
Figure 14. The release profiles of malic acid from the whey protein... 72
Figure 15. The release profiles of malic acid from the whey protein... 75
Figure 16. The migration profile of malic acid in the chicken breast processed meat sample coated with the whey... 78
Figure 17. The migration profile of malic acid in the chicken breast processed meat sample coated with the cold... 79
Figure 18. Average concentrations of malic acid (MA) in the whey protein isolate film incorporating malic acid... 80