Title Page
Contents
ABSTRACT 11
Ⅰ. Introduction 13
Ⅱ. MATERIALS AND METHODS 18
2.1. Production of artificial biofilms 18
2.2. Transmittance characteristics 20
2.3. Field-emission scanning electron microscopy images of artificial biofilms 21
2.4. Water content in artificial biofilms 21
2.5. Measurement of hydrogen production 22
2.6. Live/dead cell staining 24
Ⅲ. RESULTS & DISCUSSION 25
3.1. Structural Characterization of Artificial Biofilms by Electrophoresis 25
3.1.1. Artificial films produced by EPD 25
3.1.2. Transmittance of artificial biofilms 27
3.1.3. Structural characteristics of artificial biofilms 29
3.1.4. Water content of artificial biofilms 31
3.2. Biological characterization of bacteria inside artificial biofilms 32
3.2.1. Hydrogen generation from artificial biofilms 32
3.2.2. Survival rate of bacteria in artificial biofilms 35
3.2.3. Long-term hydrogen generation from artificial biofilms with substrate replacement 37
Ⅳ. CONCLUSION 39
Ⅴ. REFERENCES 41
ABSTRACT IN KOREAN 47
Table 1. Experimental groups for hydrogen generation. 33
Figure 1. Fabrication process of artificial biofilms composed of sodium alginate and Rhodospirillum rubrum using electrophoretic deposition. 19
Figure 2. Hydrogen production experimental cases. 23
Figure 3. Artificial biofilms produced by EPD. 26
Figure 4. Light transmission of artificial biofilms. 28
Figure 5. Scanning electron microscopy (SEM) images of artificial biofilms. 30
Figure 6. Water content of artificial biofilms. 31
Figure 7. Hydrogen generation from artificial biofilms. 34
Figure 8. Survival rates of R. rubrum in artificial biofilms before and after hydrogen generation. 36
Figure 9. Long-term hydrogen production with periodic substrate change. 38