Title Page
Contents
ABSTRACT 9
Ⅰ. INTRODUCTION 11
Ⅱ. MATERIALS AND MATHODS 14
2.1. Cell culture 14
2.2. Fabrication of Endothelial Cell derived Nanovesicles (EC-NVs) 14
2.3. Characterization of EC-NVs 15
2.4. qRT-PCR 15
2.5. Osteogenic differentiation of adipose-derived stem cells (ADSCs) 16
2.6. Three-dimensional (3D) cell culture in hydrogel 17
2.7. Immunofluorescence staining 18
2.8. Statistical analysis 18
Ⅲ. RESULTS 19
3.1. Characterizations of EC-NVs 19
3.2. Cytotoxicity and proliferation of EC-NVs 21
3.3. EC-NVs enhanced osteogenesis in ADSCs 23
3.4. EC-NVs accelerated angiogenesis in ADSCs. 27
3.5. Osteogenic and angiogenic effect of EC-NVs was confirmed on 3D hydrogel culture. 31
3.6. miRNA microarray assay was conducted. 34
Ⅳ. DISCUSSION 37
Ⅴ. REFERENCES 39
Ⅵ. ABSTRACT IN KOREAN 42
Figure 1. Schematic illustration of ADSCs treated with EC-NVs for vascularized bone regeneration. 13
Figure 2. Characterization of EC-NVs 20
Figure 3. Cellular responses of EC-NVs to ADSCs. 22
Figure 4. Osteogenic differentiation in ADSCs treated with EC-NVs 24
Figure 5. Osteogenic differentiation characteristics of ADSCs treated with EC-NVs. 26
Figure 6. Induction of angiogenic differentiation in ADSCs treated EC-NVs. 28
Figure 7. Angiogenic effect of EC-NVs. 30
Figure 8. Osteogenic and Angiogenic co-differentiation of ADSCs treated with EC-NVs in 3D hydrogel culture 33
Figure 9. miRNA microarray of EC-NVs 36