Title Page
Contents
ABSTRACT 8
Ⅰ. INTRODUCTION 10
Ⅱ. MATERIALS & METHODS 14
1. Cell culture and morphology observations 14
2. Drug treatment 14
3. Immunoblotting 15
4. RNA extraction and quantitative real-time polymerase chain reaction 16
5. Lysosome isolation 17
6. Lysotracker staining and microscopy 17
7. Co-immunoprecipitation 18
8. Immunofluorescence analysis 18
9. Statistical analysis 19
Ⅲ. RESULTS 20
1. Effect of TRIP-Br1 on Staurosporine (STS)-mediated apoptosis and necrosis/necroptosis 20
2. Impact of TRIP-Br1 on expression of lysosomal hydrolases 23
3. Effect of TRIP-Br1 on Galectin-3 and TRIM16 27
4. Effect of TRIP-Br1 on lysophagy, autophagy-mediated damaged lysosomal removal 33
Ⅳ. DISCUSSION 38
Ⅴ. REFERENCE 42
Ⅵ. ABSTRACT IN KOREAN 44
Figure 1. Effect of TRIP-Br1 on staurosporine (STS)-mediated apoptosis and necrosis/necroptosis 22
Figure 2. Impact of TRIP-Br1 on expression of lysosomal hydrolases 26
Figure 3. Effect of TRIP-Br1 on Galectin-3 and TRIM16 32
Figure 4. Effect of TRIP-Br1 on lysophagy, autophagy-mediated damaged lysosomal removal 37
Figure 5. Summary model 41