Title Page
Contents
ABBREVIATIONS 8
ABSTRACT IN KOREAN 10
Ⅰ. INTRODUCTION 12
Ⅱ. MATERIALS AND METHODS 15
1. Preparation of green tea extracts 15
2. LC/MS chromatography 15
3. Cell culture 17
4. Western blot analysis 18
5. RNA extraction and quantitative RT-PCR 20
6. Immunostaining, H&E staining, and image analysis 21
7. Animal studies 23
8. Statistics 23
Ⅲ. RESULTS 24
1. GTE promotes myogenesis in C2C12 myoblast 24
2. GTE alleviates atrophy in C2C12 myotube 31
3. GTE affects myogenesis in human primary skeletal muscle cells 36
4. GTE improves mitochondrial membrane potential polarity 43
5. GTE modulates early stage of muscle regeneration in CTX-induced muscle injury 47
6. GTE improves muscle regeneration in CTX-induced muscle injury 51
Ⅳ. DISCUSSION 56
Ⅴ. REFERENCES 59
ABSTRACT 63
Figure 1. GTE promotes myogenesis in C2C12 myoblast 29
Figure 2. GTE alleviates atrophy in C2C12 myotube 35
Figure 3. GTE affects myogenesis in human primary skeletal muscle cells 41
Figure 4. GTE improves mitochondrial membrane potential polarity 46
Figure 5. GTE modulates early stages of muscle regeneration in CTX-induced muscle injury 50
Figure 6. GTE improves muscle regeneration in CTX-induced muscle injury 55