Title Page
Contents
국문초록 8
ABSTRACT 10
I. Introduction 13
II. Objectives 19
III. Materials and Methods 20
1. Experimental animals and setting 20
2. Rationale of the estimation of the number of experimental animals 21
3. Dosing rationale 23
4. Creation of wounds 23
5 . Experimental procedures 26
6 . Histologic examination 29
7. Immunohistochemistry (IHC) protocol 31
8. Wstern blot analysis 33
9. Statistical analysis of the experimental data 34
VI. Results 35
1. Macroscopic examinations of the diabetic wounds 35
2. Histologic examinations 40
3. Immunohistochemical findings 45
4. Results of the western blotting analysis 51
V. Discussion 61
VI. Conclusion 68
References 69
Table 1. Criteria for calculating histologic scores. 30
Table 2. The diameter of diabetic wounds. 39
Table 3. Histologic scores. 43
Fig. 1. Creation of the diabetic wounds in C57BL/6 mice. 25
Fig. 2. Experimental schema. 28
Fig. 3. Macroscopic examinations of the diabetic wounds at different time points. 36
Fig. 4. Time - dependent changes in the diameter of diabetic wounds at 3, 7, 10 and 14 days. 38
Fig. 5. Histologic findings. 41
Fig. 6. Histologic scores. 44
Fig. 7. Representative immunohistochemical VEGF staining of skin wounds samples between each group. 46
Fig. 8. Representative immunohistochemical TGF -β1 staining of skin wounds samples between each group. 49
Fig. 9. Results of the western blot analysis - VEGF 52
Fig. 10. Results of the western blotanalysis - TGF -β1 55
Fig. 11. Results of the western blot analysis - Collagen type I 58
Fig. 12. Results of the western blot analysis - Collagen type III 60