표제지
국문 요약
목차
I. 서론 10
1. 연구의 필요성 및 목적 10
2. 연구의 가설 13
1. 행동 검사 13
2. 도파민 13
3. 흑질 내 염증 수치 13
4. 선조체 내 염증 수치 13
II. 연구 방법 14
1. 동물실험설계 14
2. 파킨슨 질환 유도 마우스 모델 14
3. 운동방법 15
4. 분석 방법 16
1) Rota rod test 16
2) Nest building test 16
3) 조직 적출 17
4) Western Blot analysis 17
5) Immunohistochemistry 18
5. 통계 처리 방법 19
III. 연구 결과 20
1. Rota rod test(RRT) 20
2. Nest building test 22
3. 집단 간 흑질 조직 내 DAT, TH 발현수준 차이 24
4. 집단 간 선조체 조직 내 DAT, TH 발현수준 차이 26
5. 집단 간 흑질 조직 내 TNF-α, IL-6,IL-1β 발현수준 차이 28
6. 집단 간 선조체 조직 내 TNF-α, IL-6, IL-1β 발현수준 차이 30
7. 집단 간 흑질 조직 내 IL-10 발현수준 차이 32
8. 집단 간 선조체 조직 내 IL-10 발현수준 차이 33
IV. 논의 34
1. Rota road test 및 Nest building test 34
2. 도파민 수준 35
3. 염증 수준과의 관계 35
V. 결론 37
참고문헌 38
ABSTRACT 44
Table 1. Ladder Climbing Exercise protocol 15
Table 2. Nest building test Rating Scale 17
Table 3. The result of ANOVA on Rota rod test among groups. 21
Table 4. The result of ANOVA on Nest building test among groups. 23
Table 5. The result of ANOVA on DAT and TH among groups. 25
Table 6. The result of ANOVA on DAT and TH among groups. 27
Table 7. The result of ANOVA on TNF-α, IL-6, IL-1β among groups. 29
Table 8. The result of ANOVA on TNF-α, IL-6, IL-1β among groups. 31
Table 9. The result of ANOVA on IL-10 among groups. 32
Table 10. The result of ANOVA on IL-10 among groups. 33
Figure 1. The Effect of 8 weeks Resistance exercise on Rota-rod test on MPTP-induced mouse. (A) Time in first fall, (B) Number of the falling. 20
Figure 2. The Effect of 8 weeks Resistance exercise on Nest building test on MPTP-induced mouse. (A) Nest building score, (B) Nest of various groups. 22
Figure 3. The Effect of 8 weeks Resistance exercise on DAT, TH in the SNpc of MPTP-induced mouse. (A) Western blot Ponceau images. (B) Representative Western blot... 24
Figure 4. The Effect of 8 weeks Resistance exercise on DAT, TH in the Striatum of MPTP-induced mouse. (A) Western blot Ponceau images. (B) Representative Western blot... 26
Figure 5. The Effect of 8 weeks Resistance exercise on TNF-α, IL-6, IL-1β in the SNpc of MPTP-induced mouse. (A) Western blot Ponceau images. (B) Representative Western blot... 28
Figure 6. The Effect of 8 weeks Resistance exercise on TNF-α, IL-6, IL-1β in the Striatum of MPTP-induced mouse. (A) Western blot Ponceau images. (B) Representative Western... 30
Figure 7. The Effect of 8 weeks Resistance exercise on IL-10 in the SNpc of MPTP-induced mouse. (A) Western blot Ponceau images. (B) Representative Western blot... 32
Figure 8. The Effect of 8 weeks Resistance exercise on IL-10 in the Striatum of MPTP-induced mouse. (A) Western blot Ponceau images. (B) Representative Western blot... 33