Title Page
Contents
ABSTRACT 8
Ⅰ. INTRODUCTIONS 10
Ⅱ. EXPERIMENTAL SECTION 16
A. Materials 16
B. Preparation of GNRs 18
C. Fabrication of NFs 18
D. Characterization of NFs 19
E. Drug release at different temperatures 19
F. On-demand drug release 20
G. Analysis of biocompatibility and cellular uptake 21
H. MTT assay for cell viability 22
I. Data and statistical analysis 24
Ⅲ. RESULTS AND DISCUSSION 25
Ⅳ. CONCLUSION 45
ABBREVIATIONS 47
Reference 48
Figure 1. Schematic illustration of on-demand drug release due to glass transition from nanofibers. (a) Preparation of nanofibers containing gold... 15
Figure 2. Scanning electron microscope images of nanofibers (NFs). (a, b) Without near-infrared irradiation. (c, d) With near-infrared irradiation of 1.6... 27
Figure 3. Confocal laser scanning microscope images of nanofibers containing gold nanorods and calcein. (a) Optical image; (b) fluorescence image; (c)... 28
Figure 4. (a) Cumulative drug release from the matrix of the nanofibers (NFs) due to different temperatures. (b) Temperature changes of NFs and water... 30
Figure 5. (a) Fluorescence intensity of drug release. (b) Cumulative drug release from nanofibers (NFs) upon near-infrared irradiation with different... 32
Figure 6. (a) Rate of change in temperature of nanofibers upon cyclic on-off near-infrared irradiation (b) Drug release from the nanofibers through cyclic... 34
Figure 7. Confocal laser scanning microscope images of cell proliferation and cellular uptake on the surface of the calcein-loaded nanofibers. (a-c) Cells on... 36
Figure 8. CLSM images of Live-dead staining using FDA and PI and cell proliferation (a) 24 h and (b) 48 h before staining. 38
Figure 9. Stability analysis of nanofibers containing camptothecin and gold nanorods based on different time periods. (a-c) SEM images. (d-f)... 40
Figure 10. (a) In vitro cytotoxicity of HeLa cells with (a) nanofibers (NFs) containing gold nanorods in the absence of camptothecin (CPT) at near-... 43