In this study, comprehensive mosquito control based on mosquito collection data by ecological environment was carried out in Cheonan from June 2022 to October 2022 in order to improve the current mosquito control method that is uniformly implemented despite the very different composition of mosquito habitats, the form of occurrence sources, and the ecology. Cheonan City is a representative provincial complex city that exhibits various landscape types according to the period of urban formation, and is divided into urban and rural, old, down town, and new town according to the ecological environment landscape, and representative administrative buildings were set according to the ecological environment, and four experimental areas with a radius of 100m were installed for each area, and CO₂ (dry ice) attractant traps were installed in each experimental area to collect adult mosquitoes every week. From the second week of July, when there were more than 50 mosquitoes collected in each experimental area, comprehensive mosquito control was carried out to measure the control effect. During this study period, 16,257 mosquitoes of 5 genera and 11 species were collected from a total of 240 attractant traps, 71.93% were Culex, followed by Aedes, Ochlerotatus, Armigeres, Anopheles, and Mansonia, respectively, 14.68%, 10.32%, 3.07%, and 0.01%. In this study, the average mosquito control effect by experimental area was the highest at 45.6% in the down town, followed by 44.0% in the old town, 22.9% in the urban and rural, and 18.1% in the new town center. Compared to the existing epidemic, the number of quarantine dispatches decreased the most in the down town at 83.6%, followed by 80.7% in the new town, 71.4% in the old town, and 59.3% in the urban and rural, making it possible to derive an economical mosquito control cycle in all experimental areas. The derived mosquito control cycle is carried out once a week in urban and rural area, once every two weeks in the old town area, once every four weeks in the down town area, and once every three weeks in the new town area. This study is the first research case to test the actual mosquito control in the field, and we hope that based on the results of this study, it will be used as a basis for improving the mosquito control method of local governments and developing public quarantine policies. In addition, it is expected to help improve the response capacity of public quarantine to the outbreak of mosquito-borne infectious diseases that may enter Korea in the future and provide better quarantine services to citizens.