표제지
목차
ABSTRACT 10
Ⅰ. 서론 12
Ⅱ. 재료 및 방법 15
1. 실험재료 15
2. 세포 및 세포배양 15
3. 세포 성장 억제 분석(MTT 분석) 16
4. Cell live & dead assay 분석 16
5. Hematoxylin & Eosin 염색 16
6. 세포핵 형태 관찰 17
7. Caspase-3/-7 활성 분석 17
8. 세포 사멸 관련 단백질 발현 변화 분석 17
9. 실험자료의 통계학적 검정 18
Ⅲ. 실험결과 19
1. FaDu 세포에서 cimifugin에 의한 농도의존적 세포독성 증가 19
2. FaDu 세포에서 cimifugin에 의한 농도의존적 세포 생존율 감소 21
3. Cimifugin에 의한 FaDu 세포의 형태학적 변화 증가 23
4. Cimifugin에 의한 FaDu 세포의 염색질 응축현상 증가 25
5. FaDu 세포에서 cimifugin에 의한 caspase-3/-7의 활성 증가 27
6. FaDu 세포에서 cimifugin에 의한 외인성 및 내인성 세포사멸 29
7. FaDu 세포에서 PI3K/Akt 신호전달 경로로 매개된 cimifugin에 의한 세포 사멸 33
Ⅳ. 고찰 35
Ⅴ. 참고문헌 39
Figure 1. Chemical structure of cimifugin. 14
Figure 2. Cimifugin decreases the viability of FaDu cells, but dose not affects that of L929 cells used as a normal cells. 20
Figure 3. The survival rate is decreased by cimifugin in FaDu cells, but not in L929 cells used as a normal cells. 22
Figure 4. The formation of apoptotic bodies is increased in the FaDu cells treated with cimifugin, but is not observed in L929 cells used as a normal cells. 24
Figure 5. The number of FaDu cells with condensed chromation is increased by cimifugin. 26
Figure 6. The activity of caspase-3/-7 is increased by cimifugin in FaDu cells. 28
Figure 7. Cimifugin upregulates the expression of FasL and cleaved caspase-8, which are involved in death receptor-mediated extrinsic apoptosis in FaDu cells. 30
Figure 8. Cimifugin induces a mitrochondria-dependent intrinsic apoptosis that is mediated by the upregulation of pro-apoptotic factors and the down-regulation of... 31
Figure 9. Cimifugin increases cell death through the cleavage of caspase-3 and its down-stream pro-apoptotic factor, poly (ADP ribose) polymerases, by both... 32
Figure 10. Cimifugin-induced apoptosis is mediated by the inactivation of mitogen-activated protein kinases, NF-κB and Akt cellular signaling pathways in... 34
Figure 11. Schematic diagram of cimifugin-induced apoptosis pathways. 38