Title Page
Contents
초록 9
ABSTRACT 11
INTRODUCTION 13
MATERIALS AND METHODS 17
1. Titanium 17
2. Cell culture and differentiation 17
3. Treatment of inhibitor and SLPI 17
4. Cell proliferation assay 17
5. Western blotting 18
6. ALP staining, collagen secretion and extracellular matrix mineralization 18
7. Reverse transcription and polymerase chain reaction (RT-PCR) 19
8. Statistical Analysis 20
RESULTS 21
1. Proliferation on the SLPI-treated PDLF during differentiation 21
2. Effect of SLPI on the protein expression on the proliferation and differentiation in PDLF and MEK inhibitor treated PDLF during differentiation 21
3. ALP expression, collagen secretion and nodule formation in SLPI-treated PDLF during differentiation 22
4. Expression of non-collagenous and Col I gene in SLPI-treated PDLF during differentiation 23
5. Illustration of SLPI signaling transduction pathway in differentiation and mineralization of PDLF on the titanium surface. 23
DISCUSSION 24
REFERENCES 38
Figure 1. Viability of the SLPI-treated PDLF during differentiation on Ti discs 55
Figure 2. The expression of mitosis and differentiation related signaling molecules in SLPI-tneated PDLF during differentiation on Ti discs 55
Figure 3. The mitosis and differentiation related signaling molecules expression through the ERK1/2 and Elk-1 signaling in SLPI-treated PDLF on Ti discs 57
Figure 4. The analysis of SLPI-treated PDLF Picro-Sirius Red staining on Ti discs 58
Figure 5. The analysis of SLPI-treated PDLF ALP staining on Ti discs 58
Figure 6. The analysis of SLPI-treated PDLF Alizarin Red S staining on Ti discs 59
Figure 7. The expression of non-collagenous and collagenous genes in SLPI-treated PDLF during differentiation on Ti discs 59
Figure 8. Illustration of SLH signaling transduction pathway in differentiation and mineralization of PDLF on the titanium surface 61