Title Page
Contents
ABSTRACT 8
1. Introduction 10
2. Materials and Methods 14
2.1. Plasmid constructs and E. coli strains 14
2.2. Synthesis of synthesized products 25
2.3. Analysis of synthesized products 26
3. Results 27
3.1. Synthesis of Phenazine-1-carboxylic acid 27
3.2. Synthesis of 1-Hydoxy-phenazine 32
3.3. Synthesis of 1-Hydoxy-phenazine-glucoside 35
4. Discussion 39
5. Conclusion 41
References 42
ABSTRACT (in Korean) 48
Table 1. Primers used in this study are listed. 17
Table 2. Plasmids and strains used in the present study 18
Figure 1. Biosynthesis scheme for PCA, 1-OH-PHZ and 1-OH-PHZ-GLC in Escherichia coli. Red coloring indicates the pathway engineered for the synthesis of... 11
Figure 2. Constructs used in this study. 16
Figure 3. HPLC analysis of the reaction product from the E. coli strain B-PCA-1~6. (a) HPLC profile of the reaction products in B-PCA-1 strain; (b) HPLC profile of the... 28
Figure 4. Synthesis of PCA using different E. coli strain. 30
Figure 5. Optimization of shikimate pathway module constructs for PCA production. 31
Figure 6. HPLC analysis of the reaction product by feeding PCA from the E. coli strain B-OHPHZ-1 and B-OHPHZ-2. (a) HPLC profile of the reaction... 33
Figure 7. Optimization of shikimate pathway module constructs for 1-OH-PHZ production. 34
Figure 8. Screening of O-glucosyltransferases for synthesis of 1-OH-PHZ-GLC. HPLC analysis of the reaction product by feeding 1-OH-PHZ from the E. coli strain... 36
Figure 9. Optimization of shikimate pathway module constructs for 1-OH-PHZ-GLC production. 38